Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

Does anyone work with femoral and tibia radiography on rats or mices?

I would like to know what protocol followed for animals undergoing some treatment that induces some type of osteopenia / loss of bone density.

13 July 2019 1,731 2 View

How can I stain cells in culture supernatant ?

I am doing endothelial cells culture and it seems I have erythrocytes contamination but I want to check that. How can I do it as these cells are only in the supernatant ? Thank you in advance

17 June 2018 1,933 4 View

What are the best research-based tools to teach pronunciation of L2 segments to second language learners?

Last year I attended an International Conference at the University of Utah about the teaching of pronunciation in second language learning and teaching. I notice that there was a general...

23 May 2018 5,599 2 View

How is your research likely to improve the approach to measuring performance quality?

I would be very interested in reading your publications related to measuring health and safety/ quality/ operations management performance - especially those related to recommendations for...

17 February 2017 7,501 2 View

Statistical Data: Experimental Design (teaching, how many students, control and normal group, definite conclusions)?

An innovative proposal, so say a new way of presenting contents or a small book with innovative ways of creating reasoning patterns: What do I need to have to prove to others, in a fully...

30 May 2016 9,386 3 View

Can you suggest a good assay to determine paracellular permeability in epithelial cells?

I am establishing a bovine oviductal epithelial cells culture using inserts of 0.4um pores and I am trying to get the cells polarized with this system. I would like to use the permeability assay...

16 January 2014 7,820 18 View

Does anyone know how test strips measure current for glucose detection?

Do they work by lateral flow and if not is it possible to make a lateral flow device with something like a test strip?

23 October 2013 7,767 4 View

How would you teach occupational safety and health management to social science classes compared to/contrasted with engineering classes?

I am planning to teach OSH to management students for the first time. I have had much experience teaching this field to technical students (engineering students, operators, etc.) and I am thinking...

01 January 1970 9,065 6 View

Help for interpretation ?

I did a survey and i found that 21 of people are unemployed , 14 are retired, when 24 are workers , what that means in terms of sustainability ? , the number of people that replied to my...

08 June 2020 7,785 2 View

What are the benefits of depositing a fungal culture in noted fungal culture collections?

During my service as Mycologist in Department of Medical Mycology, in Calcutta School of Tropical Medicine, we isolated many interesting fungi. But only a few of them are deposited in CMI, ATCC...

31 December 2019 8,522 4 View

What is a PhD or research project? How to do it? What are its phases and impacts with final consequences?

31 December 2019 4,440 3 View

Why a full bottle of homemade brandy spontaneously exploded?

Yesterday on December 5th, 2019 at 3:45 p. m. a full bottle of our homemade brandy on the small kitchen table on our kitchen spontaneously exploded. I am a retired university professor in...

11 December 2019 8,900 9 View

Why a full bottle of homemade brandy spontaneously exploded?

Yesterday on December 5th, 2019 at 3:45 p. m. a full bottle of our homemade brandy on the small kitchen table on our kitchen spontaneously exploded. I am a retired university professor in...

05 December 2019 1,498 9 View

How do you build a retirement age calculator on python?

hello guys, i need assistance in computing this Build a function retirement_age(PMT, i, FV, start_age) that calculates the (whole) age at which your customer can retire, if they: invest an...

08 September 2019 4,883 1 View

What is the future of the individual situation after retirement, for example financially, medical and humanitarian looks like?

What is the future of the individual situation after retirement, for example financially, medical and humanitarian looks like? You may be suffering from some illnesses and the absence of a wife...

05 June 2019 9,336 5 View

Should the government make it compulsory for citizens to save for the future?

Citizens do not have the discipline to save for retirement. They are myopic.

04 May 2019 1,373 3 View

Why should teacher be asked to retire ?

The knowledge of a teacher is the treasure and knowledge is not obsolete when he or she becomes senior. Is it necessary to retire a teacher when he or she is capable of teaching? Please share...

03 April 2019 2,571 13 View

Retirement age data for EU for last 20-25 years?

Hello Does anyone know if there's a data source out there that contains information on how retirement age has changed in EU over past 20 - 25 years ?

02 March 2019 9,417 2 View