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Questions related to Biochemical Methods
What if I don't read the plate immediately, what should I do and how do I preserve the plate? in the fridge or on the bench? and is it reliable to do so?
12 December 2013 5,721 2 View
After separating membrane proteins from the membrane environment, we are seeking robust and efficient ways to exchange detergents for X-ray scattering structural experiments. Are sizing columns or...
12 December 2013 885 1 View
I want to know is their hope for differential media based biochemical tests?
12 December 2013 6,436 4 View
I am trying to isolate miRNA from postmortem brain material to use in OpenArray analysis. I am using miRVANA kit and I did a first trial with about 50 mg of tissue, and 600 ul of Lysis buffer, the...
11 November 2013 5,598 22 View
I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?
11 November 2013 8,965 2 View
I am going to use this method to load protein hydrolysates into milk phospholipid nanoliposomes. However, I couldn't find a lot of information about this method. I'd appreciate it if someone can...
10 October 2013 2,063 3 View
I want to retrieve antigen by 1% SDS at 90 ℃. In this situation, can SDS dissolve cell membrane and cause protein loss?
10 October 2013 8,589 7 View
specially for host-pathogen PPIs.
10 October 2013 7,735 5 View
I want to check if some proteins have associated lipids by SDS-PAGE analysis (either tris-glycine or tris-tricine gel systems). I am looking for a protocol using black sudan. Has someone already...
10 October 2013 1,806 0 View
Technical problems with protein immunoprecipitation.
10 October 2013 4,436 1 View
When running a classical SOD (superoxide dismutase) assay, samples with xanthine (X) and xanthine oxidase (XOD) produce consistently low absorbance compared to the standards (X+XOD+SOD at...
10 October 2013 7,284 9 View
I am using JMP in order to find a relation between response (Y) and factors (B, CU, T, pH), with the following details: Y (Product of a biochemical reaction): I don't want neither...
10 October 2013 7,412 8 View
I have successfully used chloroperoxidase to oxidize the primary alcohol of an N-terminal serine to an aldehyde. I recently tried this on a different peptide that had a C-terminal serine (...
10 October 2013 1,120 7 View
In terms of biochemical reactions, what is the main difference between Pathway and Network? Can anyone give me some suggestions with examples?
09 September 2013 6,602 4 View
At a particular conc. I am getting different values of uptake rate in two different experiments. One is uptake experiment where 'X' is used in 10uM conc. Another is saturation kinetics from 5-50uM...
09 September 2013 6,039 7 View
I am generating astrocytes from stem cells and want to prove that they are actually postmitotic and not only quiescent precursor cells. Are there any reliable markers except for incorporation of...
09 September 2013 2,416 4 View
I want to assess the level of oxidative stress in blood. Which are the biochemical marker/markers to be targeted?
09 September 2013 3,535 16 View
I receive many requests for measurement of macroprolactin. Most of the measurement results of this prolactin samples are within the reference range. In these cases, is there some significance to...
09 September 2013 638 7 View
I need to use these extracts to destroy Varroa destructor, ectoparasite of Apis mellifera.
09 September 2013 8,423 0 View
08 September 2013 2,414 1 View
Agriculture science
08 August 2013 5,050 4 View
I am performing an experiment where i am insulting neurons with h2o2 for different time points which vary based on how long the h202 is actually active in the culture.
08 August 2013 7,129 0 View
i wish to use an ex vivo rat heart model to identify the effect of er stress inducers following treatment of the animals with an antioxidant. I need help in the buffer and perfusion composition...
07 July 2013 4,231 0 View
Please suggest HPLC method for estimation of saponin. Extraction method? estimation method? HPLC column?
07 July 2013 6,209 3 View