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Questions related to Biochemical Methods
can any one suggest to isolated the antiglycation agent?
12 December 2017 6,450 0 View
We have a Practical Exam on Enterobacteriaceae. We had urine as our sample. We planted it on MacConkey Agar and Blood Agar Plate. After 24 hours, we had no growth on MAC. So we performed biochem...
10 October 2017 1,034 5 View
I mean what biochemical tests are routinely used for identification of thermophilic bacteria in a lab such as OF, MR-VP, etc.
05 October 2017 7,929 4 View
I did culture isolate on TCBS agar and confirm with biochemical test. Then i did PCR techniques using toxR gene to detect. Therefore some reviewers think is is not the right way to identify this...
09 September 2017 9,335 3 View
I need a simple biochemical method for detection and identification of Salmonellae spp. and E-coli from food sample...
17 August 2017 8,906 9 View
08 August 2017 10,169 9 View
Proteoglycan in liquid samples was estimated by periodic Schiffs assay method (Mantle and Allen, 1978). Whether the presence of free glycosaminoglycan in the sample will interfere the proteoglycan...
07 July 2017 8,428 1 View
Dear Sir/Madam I have isolated the RNA from infected viral leaf, Can I dissolve pellet in TE buffer and store at -20 C. Will TE buffer cause any problem to...
07 July 2017 1,588 3 View
Like Superoxid dismutase For ELISA technique
16 May 2017 7,102 3 View
Hello dear scientists, I am following the method of L Wei, et al 2015 for the determination of total Triterpenoids contents but there is no information about the mechanism of reactions when we...
05 May 2017 3,854 3 View
To be sure, exlcuding PCR, is there any biochemical test or any kind of test for pyricularia? How spore production can be promoted for pyricularia? which media is good for maintenance and which...
05 May 2017 4,999 44 View
04 May 2017 6,818 3 View
We had ISO buffer that I made in 2013 in the freezer but another postdoc realized the buffer had turned brown and the Gibson Mix we made with it didn't work. I wondered if others have noticed...
26 April 2017 761 3 View
Now i'm finding peroxidase which can be use in high temperature (at least 60℃). most of company selling peroxidase, but it is Horseradish peroxidase and it can not use in temperature what i want...
10 April 2017 5,334 5 View
04 April 2017 10,197 4 View
Hi... I have done 16 S rRNA sequencing of some of my bacterial isolates and one of my isolates is showing only 90% sequence similarity with reference strain as the maximum identity. Should I...
04 April 2017 2,575 6 View
Serum Anti-Oxidant Estimation of Super Oxidase Dimutase (SOD) and Malondialdehyde(MDA) by ELISA Method. QAYEE-BIO KIT is a commercially available, Double-Antibody Sandwich Enzyme-Linked...
04 April 2017 4,825 2 View
Kindly suggest the various biochemical experiments that can be performed by the botany people.
04 April 2017 5,690 6 View
I was wondering if anyone had a protocol for testing insulin fibrillation? I was thinking just incubating a small amount, with gentle shaking, and then taking aliquots every 2 hours. However, do I...
04 April 2017 4,362 1 View
01 April 2017 7,491 5 View
When I use freeze-dryer, after freeze at -80C for 5 or 7h, drying it but there are always sample loss after drying, some of the dried sample get out of the glass tube. How to avoid the loss?
30 March 2017 829 7 View
03 March 2017 8,156 7 View
Hello everyone, I am looking for a method to normalize alcian blue OD in micromasses, with the amount of cells. Normally cells are fixed, AB is extracted with GuHcl 8M, then OD is measured. At...
03 March 2017 972 2 View
I put the concentrated GLP-1 in 4 ℃ all night long, then it precipitated. How can I resolve it? Any help will be appreciate.
03 March 2017 5,008 12 View