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Questions related to Biochemical Methods
I am trying to concentrate the secretome of E. coli using Vivaspin columns (GE). I succesfully concentrate the proteins but when running SDS-PAGE I observe pretty blurry bands and irregular...
21 June 2016 608 9 View
please do recommend
06 June 2016 5,903 0 View
How can I proceed a reaction between amino acid and an ester? preferably ethyl ester?
11 March 2016 1,597 2 View
03 March 2016 9,635 3 View
scintillation proximity assay is done, when to find out the potency of a new ligand compared to a known one to bind to their target receptors. Can anyone suggest any other technique to be used for...
12 December 2015 9,879 3 View
Hi, I am having some problem with the anti-glucosidase assay of the standard acarbose. I have used reported procedures, but still not getting the expected results. According to the protocol, I...
12 December 2015 7,169 5 View
07 December 2015 3,948 3 View
I am trying to fix a speedvac system by combining parts of old equipment. Came across a condensation trap of the model described above, but there is a button next to the freeze button that I'm not...
11 November 2015 3,046 2 View
When I compared the melting curve I got for a qPCR (SYBR green) run with that of another run done a few weeks earlier using the same primer set and identical cycling conditions, I find that the...
29 October 2015 2,349 8 View
Post-doc position available University of São Paulo Ribeirao Preto Medical School – Department of Pharmacology Ribeirao Preto – São Paulo - Brazil The project entitled "Sodium nitrate and nitrite:...
10 October 2015 6,040 0 View
A high flow hood with hotplate is the historical ad hoc solution but not ideal. Ideas? Acids are HCl and HNO3. A standard distillation setup wouldn't work since the quantity of samples (100s) and...
08 August 2015 8,292 3 View
I saw Bovine serum albumin in some article and potassium thiocyanate (KSCN) in another. Please anyone one with the right standard curve for Rhodanese assay should please share.
08 August 2015 6,440 6 View
I wish to know how to understand and remember this diagram and graph in a simplified manner. So that I can help to poor standard students at Undergraduate and Post Graduate level.
07 July 2015 1,395 1 View
I want to perform biotinylated protein pulldown under degenerative condition, but I don't know weather the streptavidin beads can resist it.
07 July 2015 1,166 8 View
I am purifying a His-tagged protein from an EColi expression system. After the purification I am doing an activity assay so it is vital that I get active protein. First, it seems that as my...
07 July 2015 6,223 8 View
reagents to be added and the calculation
07 July 2015 8,787 0 View
Hi, I am trying to remove unligated, biotinylated linkers/adapters from my DNA by running a low melting point agarose gel (0.8%). After cutting the DNA out, and leaving the unligated small sized...
07 July 2015 4,209 4 View
Although HRP lives under darkness most of its natural life, what possible biological role Energy transfer from Trp to heme can have? Can it possibly effect Fe reactivity in Compound I or II if...
06 June 2015 4,859 3 View
I am currently trying to generate a hydroxyproline standard curve but so far it hasn't worked. I am using a protocol from sigma's webpage and I have made all of my own reagents. I diluted a 1mg/mL...
06 June 2015 6,708 5 View
Hi all, I run few samples on a LTQ orbitrap Velos and I saw some PEG. Now, I want to test my samples using MALDI TOF/TOF before running them in the orbitrap again. Which matrix should work better...
05 May 2015 5,245 4 View
Hello Researcher How do I take the range of purified sample for caseinolytic activity, pH kinetics, temperature variation, etc. whether i take the range which I did for crude sample (10- 200...
05 May 2015 6,832 1 View
i have some data about a recombinant enzyme and i want to calculate my enzyme unite. i have used the ellman method for enzyme activity test. ODfirst minite= 0.35 ODseconde minite=0.8 reaction...
05 May 2015 7,061 2 View
after hydration method, next i reduce the size using extrusion method, i checked the morphology using TEM it's shown multivesicular vesicle, why it could be happen?
05 May 2015 5,670 4 View
My Supervisor always ask me to keep or maintain the A260 more than 20. Why it is so important? Can we just count it by the concentration of nucleid acid?
05 May 2015 8,704 8 View