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Questions related to Biochemical Methods
I have cut out the interested bands out with blades, cut them into piece and wash them with NF water, stored them in NF water in 4 degree overnight, redo PCR and check with agarose gel, then rerun...
04 April 2015 9,250 2 View
I have used phosphate buffer to dilute the pellet of enzyme. The enzyme became so much diluted so kindly tell me how to concentrate the enzyme within one day?
04 April 2015 3,277 6 View
Hello colleagues, As I started work with MALDI-TOF MS I wondering if it is possible to prepare and conserve MALDI slides for later uses, without read it immediately after the...
04 April 2015 8,833 11 View
i have certain queries regarding sample preparation.? http://www.merckmillipore.com/IN/en/product/Rac1-Activation-Assay-Kit,MM_NF-17-283
03 March 2015 9,727 0 View
We tried using the usual biochemical methods for sugar estimation but due to presence of seawater precipitation results which is hampering the spectrometer reading.
03 March 2015 5,182 4 View
Is that true in general?
03 March 2015 9,160 4 View
Dear RG experts, let me know any other technique
02 February 2015 8,433 6 View
We generally use the complete media containing phenol red to seed the cells in multiwell plate.Then after 24 h of culture we treat the cells with the drug containing media containing the drug and...
01 January 2015 2,783 10 View
To estimate the nutritional condition of fish and invertebrate larvae, biochemical methods are widely used. One of the most important is the RNA/DNA ratio. Since the quantity of DNA remains...
12 December 2014 2,993 7 View
Shall any body inform me by the source for these products, I appreciate his/her kind help.
11 November 2014 7,249 2 View
We are working on yeast isolates and are interested to screen high ethanol producing isolates. Since the number of isolates is quite high ( >200), it is not possible for us to analyze the whole...
11 November 2014 6,096 6 View
Does the body "recycle" oxidized ascorbic acid? If it does so, would thioredoxin reductases (alone or within the thioredoxin system) be involved?
10 October 2014 4,717 4 View
I know there have been plenty of experiments that use calmodulin as a bait to identify interacting proteins, but I am working with a family of proteins that have known calmodulin binding domains....
10 October 2014 4,810 4 View
I learned from some papers of hospitals thats some Streptococcus pneumoniae strain are not fit the “Gold characters” of most Streptococcus pneumoniae that are Optochin susceptible and Bile...
09 September 2014 9,506 1 View
Dear all How do I estimate the inflammatory mediators without using ELISA, are there any biochemical methods for estimation of inflammatory mediators.
09 September 2014 2,374 9 View
Are all Phytochromes photoreversible?
09 September 2014 7,698 1 View
I Know the E3 ligase for my protein and i am intrested to find the site of ubiquitination. In its vicinity 7-8 lysine are resent at regular interval. Does anyone have any idea?
09 September 2014 8,230 3 View
To understand the action of some microtubule target agents. Can anybody help me with a good protocol or work design to analyse depolymerisation of microtubule
17 August 2014 1,345 3 View
08 August 2014 5,771 4 View
I work with a natural adhesive (footprint) extracted from a marine organism onto the glass slide or mica. It is very cohesive sample and so far, we have detected amyloid fibrils in the footprint...
08 August 2014 4,180 3 View
What are the Biochemical methods for identifying Bifidobacterium and Lactobacillius colonies?
07 July 2014 10,071 11 View
I want to do a coagulase test for the identification of staphylococcus aureus. When I asked how to get the "rabbit plasma " and what is the most appropriate procedure for doing so, someone...
06 June 2014 1,499 7 View
Needing references published in these journals
06 June 2014 392 1 View
I wants the protocol for analyzing the SOD activity for tea leaves.
06 June 2014 1,161 1 View