I am going to use this method to load protein hydrolysates into milk phospholipid nanoliposomes. However, I couldn't find a lot of information about this method. I'd appreciate it if someone can provide me some more information on this specific topic.
HI Zhiyu
I am not sure if this is the best way to incorporate protein hydrolates into the milk. I have used mechano-encapsulation technique on various different drugs/proteins and it seems to work much better than heating method. Also, you may want to get in touch with guys at company called Evonik as they have all sorts of different options. I find them to be very useful in my research (I do not promote them but rather point you in direction)...
Kind Regards
Nenad
Hi Nenad,
Thanks you kindly for your suggestions.
I chose liposome to encapsulate protein hydrolysates because hydrolysates have high content of both hydrophobic and hydrophilic peptides. I would be appreciated if you could suggest me some other encapsulation material.
Heating method one of the method I am going to use. Meanwhile, I will use high pressure homogenization and extrusion methods.
Regards.
Zhiyu
Hi
So sorry to getting back to your question after such a long time (I just saw your question few minutes ago).
Attached article will explain "Heating Method" and its better version: "Mozafari Method" in details. Pls let me know if you require more details.
Mozafari Method steps:
1. Add the liposomal ingredients to a preheated (60°C, 5 min)
mixture of nisin (200 mg/ml) and a polyol such as glycerol
(final concentration 3%, v/v) in a heat-resistant flask such as a
pyrex beaker. Alternatively, a heat-resistant bottle with six
baffles can be used as explained and pictured in reference (49).
2. Heat the mixture at 60°C while stirring (approx. 1,000 rpm) on a
hotplate stirrer (e.g. RET basic IKAMAG1 Safety Control, IKA,
Malaysia) for a period of 45–60 min under nitrogen atmosphere.
3. For the preparation of cholesterol-containing formulations,
first dissolve the cholesterol in the aqueous phase at elevated
temperatures (c. 120°C) while stirring (approx. 1,000 rpm)
for a period of 15–30 min under nitrogen atmosphere before
adding the other phospholipid components.
4. Depending on the formulation ingredients, sample volume,
type of flask used and its number of baffles, as well as type, speed
and duration of mixing, nanometric vesicles can be produced in
one step, without the need to perform filtration or sonication.
5. Leave the nanoliposome suspension at temperatures above Tc
under an inert atmosphere such as nitrogen or argon for 1 h
to allow the sample to anneal and stabilize.
Mozafari, M.R. (2010) Nanoliposomes: preparation and analysis. Methods in Molecular Biology, 605, 29-50. ISSN 1064-3745
Article Nanoliposomes: Preparation and Analysis
- Badges
- Science method
- Similar topics
- Chemistry
- General Biochemistry
- Biochemical Methods