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Questions related to Biochemical Methods
I'm needing some papers published in this periodic/volume.
06 June 2014 687 0 View
I intend to use casein micelles as nanovehicles for the delivery of a hydrophobic compound, by emulsifying the compound solution into a caseinate solution and then adding the salts to reassemble...
05 May 2014 7,081 1 View
Needs to be directly after the signal peptide sequence so it doesn't get cleaved.
04 April 2014 5,397 0 View
I am trying to dissolve 2.3g/l of sodium hyaluronate in phosphate buffer saline. However, it is taking me two days to dissolve. I am wondering if somebody knows a way to dissolve it faster. I...
04 April 2014 8,407 6 View
I need to measure hydrogen peroxide in sea water samples, out of the lab. Does anyone know a reliable and quick method?.
04 April 2014 5,002 3 View
I use a density gradient formed from KBr. Often bands appear relatively close (1-2 mm apart) and would like to recover them separately for further analysis.
04 April 2014 7,552 4 View
I am going to perform a urease inhibitory assay on ELIZA micro plate reader. Please brief me on this.
04 April 2014 6,281 1 View
see above
03 March 2014 1,074 2 View
I want to construct an shRNA-plasmid containing multi-domains of a single gene with one hairpin and under the control of single promoter. Is it possible?
03 March 2014 7,822 1 View
I am not getting a very clear signal even though my actin bands are perfect. Is there any way to amplify the pAkt bands?
03 March 2014 7,151 7 View
I recently started using vinculin as a loading control for high molecular weight proteins. Strangely, vinculin showed up evenly despite a 2-fold difference in loading. I wonder what is happening...
02 February 2014 8,811 3 View
To obtain the correct orientation of antibodies binding to TiO2 nanostructured surface.
02 February 2014 5,027 1 View
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02 February 2014 3,844 2 View
I need to screen secondary metabolites such as Beta carotene, Flavonoid, Betalains, Anthocyanin etc. from certain yeast isolates. Hence I need some biochemical methods for preliminary investigation.
01 January 2014 8,930 1 View
It could be a potential substrate for few of them. Maybe someone could be so kind to give me a hint which of them is the most involved in utilizing 1,3-butanediol or 4-hydroxy-2-butanone (reverse...
01 January 2014 7,318 0 View
I want to conjugate a fluorescent molecule to particles I am using. My aim is to inject it to mice veins and detect it in the mice harvested organs. The fluorescent molecule needs to be stable...
01 January 2014 9,823 4 View
I am teaching a undergraduate level Biochem Lab course. The first part of the labs are protocol-based experiments (protein extraction, purification, quantification and activity measurement). The...
01 January 2014 985 4 View
I am doing the isolation of pure compounds from Moringa leaves. The problem is, the compound of the Moringa leaves has Rf adjacent, making it difficult to be separated. Please share your...
01 January 2014 5,374 4 View
I have to analyze it in olive.
01 January 2014 6,386 3 View
Which Fluid -Structure Interaction (FSI) software is best (simple, easy for analysis and requiring minimum computer hardware configuration) for the simulation of blood flow through the artery?...
01 January 2014 4,988 3 View
Colorimetric analysis will be good for my lab.
01 January 2014 9,207 8 View
How should I choose the concentration from crude extract for a new study: 1. use serial dilution 10x, 100x, 1000x of crude extract? 2. or use extract without certain concentration? is it...
01 January 2014 5,191 6 View
Can anyone suggest a protocol for DNA pull-down assays?
01 January 2014 8,348 4 View
I would like to separate the two fractions so as to probe for the presence of the protein of my interest in the different fractions.
12 December 2013 2,721 5 View