Biochemical Methods

What is the function of using LiCl in place of NaCl, in one of the washes of ChIP?

With the ChIP protocol there are two washes performed, one with a buffer that has the following ingredients: Tris-HCl pH 8 EDTA NaCl Triton X-100 SDS and the other which has the...

09 September 2012 4,303 3 View

How to best validate the smoking status of adolescents?

Dear Colleagues, we are currently conducting a randomized trial with 10,000 students and feel the need to validate their self-reported cigarette smoking status via a biochemical method. I came...

01 January 1970 1,444 9 View

What is the biochemical tests that characterize the Streptococcus thermophius?

What is the shape and arrangement of streptococcus thermoplilus under microscope?

01 January 1970 2,333 1 View

What is the role of chloroform, isopropanol, as well as NP-40 during the extraction of cholesterol from lysed cells?

I want to determine whether the centrifugation, separation of organic phase, & chloroform: Isopropanol: NP-40 steps are necessary in sample prep as per cell biolabs Cholesterol Assay (using...

01 January 1970 1,598 3 View

Does anibody knows if the Anti-beta Amyloid 1-42 antibody reacts with extracleular peptyde or only with intracelular?

I want to see if peptydes are going to build plaques.

01 January 1970 7,035 4 View

Can you find antioxidants in extract and fraction from Galega officinalis by this method?

Extracts from G. officnalis supress platelet aggregation

01 January 1970 5,926 1 View

How to validate the cigarette smoking status of adolescents?

Dear Colleagues, I asked this question in a modified way before. Please read this text carefully before giving an answer. Let me provide you with some background: We currently run two major...

01 January 1970 8,136 3 View

I need a kits for isolation of glycated hemoglubin and non gelycated proteins from the blood ?

i am working in HbA1c biosensor modifications, and i need a protein standard for my experiments both glycated and non-glycated. i need a kit or manual procedure for the isolation of protein to use...

01 January 1970 488 2 View

How reliable are biochemical tests for quantification of unknown compound?

I want to determine the concentration of a flavonoid molecule. The error due to weighing is quite high as the amount of sample is less. So, I thought of trying biochemical tests like Aluminium...

01 January 1970 7,963 3 View

Investigation of bioactive secondary metabolites of Boraginaceae species

any biochemical method of investigating

01 January 1970 5,978 1 View

Hi Dear Melyssa with kind which biochemical test you do it in this project ? with my thanks Dr.kalthum A.Maulood

biochemical test ,

01 January 1970 8,207 0 View

Specific growth medium and Various Biochemical Tests that are to be performed such a way that it shows positive results for the food pathogens ??

May I know about the specific growth medium and  what are the Biochemical tests showing positive results for the following species 1. E.coli O157:H7 2. salmonella typhimurium 3. Staphylococcus...

01 January 1970 8,445 7 View

What kind of variables are you looking for to determine the frailty?

I mean about medical variables (Blood and biochemical tests,..) or movement and biomechanical variables (as gait speed, strength of limbs, coordination,..)? I think that you are making the...

01 January 1970 4,494 0 View

Do you need any help for achieving tests?

ELISA_ Antioxidants_biochemical parameters We have ability to make diagnostic biochemical tests on sera and tissues for researchers and other clients

01 January 1970 9,455 3 View

Help me out in identifying this bacteria

Biochemical test result presented the isolate as MR +ve VP +ve citrate -ve oxidase +ve catalase -ve indole...

01 January 1970 1,543 1 View

No title

01 January 1970 8,500 0 View

How reliable are biochemical tests for quantification of unknown compound?

I want to determine the concentration of a flavonoid molecule. The error due to weighing is quite high as the amount of sample is less. So, I thought of trying biochemical tests like Aluminium...

01 January 1970 2,449 4 View

Do you need any help for achieving tests?

ELISA_ Antioxidants_biochemical parameters We have ability to make diagnostic biochemical tests on sera and tissues for researchers and other clients

01 January 1970 7,552 3 View

No title

01 January 1970 264 0 View

I need a kits for isolation of glycated hemoglubin and non gelycated proteins from the blood ?

i am working in HbA1c biosensor modifications, and i need a protein standard for my experiments both glycated and non-glycated. i need a kit or manual procedure for the isolation of protein to use...

01 January 1970 2,466 2 View

Does anyone know a method/protocol to allow bacterial ribosome subunits to ri-associate?

I am purifying 30s and 50s ribosome subunits to successively perform studies on their ri-association upon inhibitor addition. 

01 January 1970 2,229 5 View

Does anibody knows if the Anti-beta Amyloid 1-42 antibody reacts with extracleular peptyde or only with intracelular?

I want to see if peptydes are going to build plaques.

01 January 1970 8,227 3 View

What is the role of chloroform, isopropanol, as well as NP-40 during the extraction of cholesterol from lysed cells?

I want to determine whether the centrifugation, separation of organic phase, & chloroform: Isopropanol: NP-40 steps are necessary in sample prep as per cell biolabs Cholesterol Assay (using...

01 January 1970 6,000 3 View

Biochemical assay: quercetin preparation?

Why my quercetin left precipitate when I did serial dilution for alpha-glucosidase assay?

01 January 1970 5,588 3 View