I am performing an experiment where i am insulting neurons with h2o2 for different time points which vary based on how long the h202 is actually active in the culture.
I've isolated primary microglia using magnetic microbeads, however the cells I've isolated don't seem to be growing very well. So, i'm cautious about how useful they would be in experiments,...
06 July 2014 1,752 13 View
Upon treatment the cells differentiate to neurons within 48 hours. Is there a method to delay this response long enough to complete treatment and isolation for RNA extraction?
11 December 2013 3,347 0 View
I am analyzing PCR arrays for several pathways in neural stem cells at about 84 genes each. The only software I've seen available for pathway analysis is Ingenuity which is 500 dollars per report....
11 December 2013 8,636 1 View
I am looking to measure TCF/LEF vs FOXO activation by beta catenin and would like to use q rt PCR but I don't know what the standard target genes are that I could reliably probe for.
08 September 2013 1,828 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View