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Questions related to Biochemical Methods
I need to isolate early endosomes in a easy fashion. It is possible?
07 July 2013 510 0 View
Collection of DNA-protein conjugates
06 June 2013 2,111 0 View
I have a protein stock that is stored in 1.5 mL eppendorf tubes that I originally (approx 1 year ago) aliquoted at 300mL/tube. I'm curious if kept in the closed tubes for long term storage, can...
06 June 2013 4,183 26 View
I was wondering if anybody has ever used the Nano Drop 2000c to measure fluorescence emission or excitation of a protein activity assay? Basically, I want to assess the activity of my protein...
06 June 2013 6,306 1 View
I plan to remove all glycans from a 250KD glycoproteins and then use the deglycosylated protein as an antigen to do western blot . In fact, I do not have this purified glycoprotein. My material...
06 June 2013 4,325 11 View
What do you think about this technique for quantitative ligand binding assays?
06 June 2013 8,818 2 View
A method for detection of his-tagged target biomolecule while said molecule remains in the gel in which it has been separated from other constituents by electrophoresis.
06 June 2013 581 3 View
I am attempting an extraction of garlic poly sulfides from fresh, aged, or boiled garlic samples suspended in water. (5 grams chopped bulb in 5 ml water) Two pulls with 5 ml chloroform yielded a...
05 May 2013 4,223 7 View
I want to quantify the amount of ascorbic acid from leaf tissue of Arabidopsis for quantification of level antioxidants in transgenic plant.
05 May 2013 6,649 0 View
I am trying to label a membrane protein with MTSSL (a thio specific spin label). The Membrane protein is a single cysteine mutants at different positions for EPR measurements. The Protein is in...
04 April 2013 3,617 7 View
Is it worth publishing in such open excess journals, most of which are not even indexed. What about their credibility in terms of research dissemination or they are mere introduced to earn bucks...
03 March 2013 7,369 18 View
Why is sucrose used for isotonic solution preparation? Why not any other mono or disaccharides, like glucose, maltose or fructose?
02 February 2013 861 3 View
I have started a project to share protocols and methodological details on amyloids. For now, it would be located on this site and be open to anyone who wants to join (don't put proprietary...
02 February 2013 2,249 71 View
I will use caspase-3 kit (BML-AK700 Enzolife) for rat brain hippocampus and retina tissues. But I don't know which solution I should use for tissue homogenization, PBS or another solution? I...
02 February 2013 722 1 View
I am making a total protein extract from a bacterium. In the final buffer I diluted the sample with 7M Urea, 2M Thiourea, 4% NP40, Tris, PMSF, EDTA and DTT. I have tried to quantify the sample...
01 January 2013 9,430 10 View
I am extracting surface proteins of G(+) bacteria by 5M LiCl extraction (Toba et al., 1995; Turner et al., 1997). I try to concentrate my samples by precipitation with Sigma-Aldrich ProteoPrep...
01 January 2013 5,037 13 View
I'm tryng to detect peptide-SH3 domain interaction by using the Thermo GSH coated microplates, but our results are not consistent. Perhaps the buffers we are using are not the best? And how can I...
01 January 2013 6,502 0 View
I´m working with an alpha proteobacteira. In specific growth condition I detected, using the CAS assay, production of a chelating agent. I performed the classical chemical test to identify the...
11 November 2012 2,234 8 View
For example cyclisation of diphenyl amines to indole derivatives.
10 October 2012 3,605 0 View
How can I replace Aromatic attached SMe group with amines?
10 October 2012 2,991 15 View
With the ChIP protocol there are two washes performed, one with a buffer that has the following ingredients: Tris-HCl pH 8 EDTA NaCl Triton X-100 SDS and the other which has the...
20 September 2012 9,415 3 View
I prepare small aliquots in water at 100mM and then keep them at -20°C till my experiments. But I don't really know how long these aliquots can be kept for... Another point, from thawing to the...
14 September 2012 8,918 2 View
The ChIP protocol I'm following has a low salt, high salt, LiCl and 1X TE washes, respectively.The low salt wash buffer has 150mM of NaCl, the high salt wash buffer has 500mM of NaCl and LiCl wash...
09 September 2012 5,109 0 View
09 September 2012 1,608 2 View