I want to check if some proteins have associated lipids by SDS-PAGE analysis (either tris-glycine or tris-tricine gel systems). I am looking for a protocol using black sudan. Has someone already used such a stain? Is there another way to proceed?
Could you advise me on a simple technic for DNA sequence & termination definition (characterization) ? (I am non-biologist). Which parameters should I take in consideration ? Thank you
26 May 2024 1,590 3 View
I try to understand and identify my dsDNA structure. I need to know if my DNA has a special structure and it is double-stranded or not. Which method should I use ? Are FTIR and CD sufficient ? I...
26 May 2024 5,696 1 View
Hello everyone, I'm doing my master's thesis right now and planning to use Response Surface Modeling. The plan is to test how the (in-)congruence of a personality trait of two people affects...
20 May 2024 9,811 0 View
For example: If the data is a song. Is the sentence analysis a line-by-line?
28 April 2024 7,251 0 View
Hello Everyone! I use Golden Gate technology to build plasmids in my lab. I have a plasmid that I've built that I'm now trying to maxi prep. I've already done a miniprep on this plasmid and sent...
22 April 2024 6,700 8 View
This question is about the article "Tenets of Specimen Management in Diagnostic Microbiology," written by Rajeshwar Reddy Kasarla and Laxmi Pathak in 2023. The question centers on the advantages...
10 April 2024 2,118 2 View
According to the study "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory", most clinical chemistry and immunoassay tests can be performed...
09 April 2024 6,395 1 View
This question is derived from the journal article “The Transition from Gel Separatory Serum to Lithium Heparin Gel Tubes in the Clinical Laboratory,” written by Oguzhan Zengi. The journal...
07 April 2024 1,838 1 View
Given that blood is predominantly utilized in laboratory testing, I'm interested in exploring alternative sample sources for situations where obtaining blood from the patient proves challenging.
06 April 2024 226 2 View
We are currently doing community work where we have to measure the height and weight of children 6-10 years old. The community work is done across different communities. Thus, since the...
03 April 2024 7,311 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View