How to preserve the precipitates of an MTT assay if I'm not going to read the plate immediately?

More Laila Ziko's questions See All

Is there a mobile phase to separate acetyl-coA, malonyl co-A from free co-enzyme A on TLC? Thank you!

I need to separate acetyl co-A, malonyl co-A & free co-enzyme A on TLC, I tried several mobile phases but they didn't work with acetyl co-A, it didn't move. I tried: ethyl acetate, methanol...

06 July 2017 3,599 1 View

What is the best method to sequence Fosmid DNA?

Hi, I have a fosmid of size ~40 kb, what would be the best method to sequence it & capture all the insert?

09 October 2016 8,222 3 View

How can I extract fosmid DNA without getting multiple bands?

I need to send Fosmid DNA for sequencing, but they told me the concentrations were too low & there are multiple bands. I tried mini-prep Qiagen kit many times & also midi-prep. How can...

07 August 2016 8,161 2 View

Is this a protein extract protocol? ptns vs. metabolites?

(Felczykowska et al., 2014): Overnight cultures from E. coli were centrifuged at 3,500 rpm for 10 min. Afterwards, the cell pellets were re-suspended in 0.01 M Tris-HCl pH 7. Finally the extracts...

05 June 2016 2,529 0 View

Using bbduk for decontaminating sequences? I did Mi-seq for fosmid DNA but got some E. coli sequences contaminating the data, how can I remove them?

Using bbduk for decontaminating sequences? I did Mi-seq for fosmid DNA but got some E. coli sequences contaminating the data, how can I remove them? Someone recommended I use bbduk for this, but I...

01 January 1970 2,421 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Yosif El-Darawish

I don't think it's a good idea to wait long time before you measure the OD.

Still the color is stable for a few hours at room temperature.

Check that:

http://www.ncbi.nlm.nih.gov/pubmed/?term=Rapid+Colorimetric+Assay+for+Cellular+Growth+and++Survival%3A+Application+to+Proliferation+and++Cytotoxicity+Assays

I hope it'll help you

Laila Ziko

Thanks a lot, fortunately I didn't have to do that at the end, it was an emergency.