For saponin extraction----Use Methanol for extraction partioning with saturated butanol followed by column chrm dianionHP- 20 followed by C-18

For detection on HPLC use ACN:Water column C-18 at 210nm at PDA or you can use ELSD detector.

You can see our paper on saponins.

Gudluck

:)

Dear Robin,

thanks a lot for you valuable suggestion. i got that paper

Hi Robin,

You can also check this paper for the extraction and quantification of saponin using HPLC.

Gnoatto SC, Schenkel EP, Bassani VL. HPLC Method to assay total saponins in Ilex paraguariensis aqueous extract. J Braz Chem Soc. 2005;16:723–6.

copy the link below to check the paper.

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000500007

I hope this helps.