Please suggest HPLC method for estimation of saponin.
Extraction method?
estimation method?
HPLC column?
For saponin extraction----Use Methanol for extraction partioning with saturated butanol followed by column chrm dianionHP- 20 followed by C-18
For detection on HPLC use ACN:Water column C-18 at 210nm at PDA or you can use ELSD detector.
You can see our paper on saponins.
Gudluck
:)
Dear Robin,
thanks a lot for you valuable suggestion. i got that paper
Hi Robin,
You can also check this paper for the extraction and quantification of saponin using HPLC.
Gnoatto SC, Schenkel EP, Bassani VL. HPLC Method to assay total saponins in Ilex paraguariensis aqueous extract. J Braz Chem Soc. 2005;16:723–6.
copy the link below to check the paper.
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000500007
I hope this helps.
07 August 2017 3,484 23 View
i want to estimate the saponin content in crude extract of aloe vera using HPLC. which standard of saponin should i use to estimate the saponin or which type of saponin is found in Aloe vera?
02 March 2016 3,705 3 View
07 August 2015 8,911 7 View
I am using Biorad sequi gen sequencing gel apparatus for AFLP. In which one a plate is treated with Bind silane. After staining this is very difficult to remove the gel from the glass plate....
05 June 2014 4,901 4 View
We have sequenced the plant transcriptome using next generation sequencing. Now we have to Design SSR markers from this data. Please suggest how to design these markers.
03 April 2014 1,532 5 View
Seeking a suggestion of any DNA isolation method for different fungal strains. Some methods are working for particular strains. But I want a method which works for different strains and also best...
07 August 2013 3,288 1 View
I have synthesized cDNA from RNA (extracted by LiCl method) using Thermo RevertAid cDNA synthesis kit. The 260/280 ratio for all the cDNA samples is falling in the range 1.6-1.7 while it should be...
05 June 2013 2,124 5 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
01 March 2021 3,905 5 View
I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...
01 March 2021 1,587 6 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...
25 February 2021 2,388 3 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View