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Questions related to Annealing
I did a temperature gradient PCR where I got specific band at 68 degrees.The extension temperature is also 68 degrees. When I run the product on gel I got a less intense amplicon for 68 degrees...
27 February 2020 9,336 1 View
27 February 2020 3,141 1 View
HEMT FABRICATION.
25 February 2020 5,266 3 View
Dear All, I have set up a 5-plex qPCR. The multiplex works fine but the PCR efficiency doesn't look that great. I am throwing in 250ng human genomic DNA and I get Ct in range of 30-35 for all my...
14 February 2020 7,418 3 View
Hello Dear Researchers, I have a problem with the synthesis of the PMMA/PVP blend. I used the chloroform/methanol (90%/10%) as a mixed solvent and the sample annealed at 80C. but after annealing...
08 February 2020 5,891 4 View
Tio2 powder is earned after sulfate process by solving illmanite in the sulfuric acid and a purity of up 95% of TiO2 is gained . why the color of Tio2 turns yellow after 2h annealing in 900 oC...
08 February 2020 6,160 3 View
I'm trying to transfer a monolayer of CVD grown hBN from Cu foil to a target substrate ( in this case, a Si substrate with micro wells. )To improve the adhesion between hBN and So substrate, I'm...
07 February 2020 8,209 1 View
Hello folks, I am trying to cut a specific RNA using RNAse H. To do that I'm annealing an oligo which targets my RNA (annealing buffer TRIS 10mM, NaCl 50mM, 1mM EDTA) heating the mix (with...
04 February 2020 6,999 2 View
I'm conducting compression tests on shape memory alloy rods. can the same rod be compressed using different strain values and between each compression process its heated above its austenite finish...
04 February 2020 1,384 2 View
I would like to make the Al5083 micro structure homogeneous before deforming. In which temperature should it be annealed? Thanks.
04 February 2020 9,408 4 View
I have recently electrospun PC and ABS nanofibers and I would like to know if annealing them at different durations and temperature ranges would increase the mechanical properties.
03 February 2020 7,290 3 View
I am evaluating if annealing barium titanate in vacuum could have any benefits.
28 January 2020 4,654 1 View
I trying to amplify N40 ssDNA library with hot start polymerase, but template was not amplified. I tried different combination of cycle number, annealing temperature and time of extension and...
18 January 2020 6,504 4 View
I got confused by the shift of the absorbance region which lead to the shift of bandgap.
06 January 2020 9,263 4 View
I have developed High Entropy Alloy Coatings by ball milling of Elemental Powders i.e. AlNiCoCrFe and using Atmospheric Plasma Spraying technique. Want to know about Annealing parameters and other...
25 December 2019 8,299 2 View
TiO2/PVP with various PVP concentration were prepared. The diameter of the fiber increase with increase in PVP. What would be the effect on these diameter fiber when they will be calined? What...
21 December 2019 6,723 1 View
I am considering mainly about conductivity and trasmittance. If annealing in vacuum is better, why is it?
18 November 2019 2,138 3 View
I am TiO2 thin film using rapid thermal annealing. I have varied the annealing temperature and annealing time as well as ambient. For temperature, peaks are increased but during varying the...
13 November 2019 1,741 3 View
Hi. I did intercalation using NMP and crystal is attatched to the substrate using mechanical exfoliation. I did annealing after exfoliation so that crystal is attached to the substrate well. But...
28 October 2019 9,902 4 View
Generally coercivity, Hc found to be decrease after annealing as grain size is more. But, if it is showing increased after annealing of my metallic ball milled powder sample, what would be the...
23 October 2019 8,178 3 View
10 October 2019 6,712 3 View
10 October 2019 4,565 4 View
samples were exposed to 400°C for 6 hours.They turned golden colour after heat treatment
10 October 2019 4,696 3 View
Will carbide phases get dissolved at this temp.? What can be the probable cooling rate? Please suggest
11 September 2019 5,501 4 View