For Rt PCR?

Is it necessary to run the Rt PCR product on agarose gel after completion?

29 December 2020 4,823 3 View

How to interpret MSWEP 3-hourly rainfall data 0.1 degree resolution?

I have downloaded the MSWEP sub-daily rainfall data (3-hourly data 0.1 degree resolution). I used MATLAB to convert it and obtained a table of dimensions 1800 x 3600 for a specific date. I am...

18 October 2020 4,205 4 View

Fan boundary condition using Ansys Fluent?

I am trying to model a jet fan being installed in a room. I want to study the flow of the fan coming out of the outlet (basically the development of streamlines). The walls of the room are...

12 October 2020 4,970 3 View

In Abaqus- How to optimize the shape of a particular region while keeping displacement at two points equal?

I am new to abaqus and I am trying to optimize a shape of a segment of a model (As a part of my master thesis). Please refer to the example model I have attached. I need to optimize the segment...

23 August 2020 5,828 9 View

Shall I submit the article simultaneously to another journal even after request to first journal to withdraw the manuscript???

I have submitted one of my review article on COVID 19 and ophthalmology in April through online submission but journal haven't overlooked it inspite of several request. Even i have sent request...

06 August 2020 1,802 3 View

How do you transfer files from Open Foam to Star CCM ?

Hi I am trying to find some information as how to link up OpenFoam and Star CCM, however I am not able to find adequate information. Below is the details of the project. 1- Objective: to...

05 June 2020 8,484 3 View

How to start jupyter notebook from a particular folder instead of default root folder?

I am using python 3.6.8 on a Windows 10 machine. We can only move to subdirectories from the default installation (root) folder in jupyter. I wanted to start the notebook from a particular...

08 March 2020 9,259 4 View

Best resources for Multivariate Kriging for deterministic simulation with Python ?

Hello, I am new to surrogate-based data analysis. In my model, Input space consists 6 variables and a single ouput is noted from each simulation. I have N such simulations. ( Sampling method is...

09 February 2020 7,511 3 View

What might be the maximum time of immersion required for the sea water ageing of glass fiber reinforced polyester composites?

I am investigating the mechanical and physical property changes in the seawater aged GFRP. I am requiring a suggestion of to what maximum number of days should I age it. Is there a particular time...

30 January 2020 570 4 View

Heat Transfer to a stationary fluid through a heated tank shell, fea OR cfd?

Dear All I have a stationary volume of fluid contained in a tank shell. The fluid in the tank shell needs to be maintained at a certain temperature. The tank shell is heated up through electric...

13 January 2020 8,196 3 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

What are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems?

anyone suggest what are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems

21 January 2021 5,135 5 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View