DNA agarose gels without a dark room?

Hi. Unfortunately our lab doesn't have a dark room, so when we are cutting bands from our Sybr-Safe or Midori Green stained gels, we often find it difficult to see the bands present which are...

06 June 2019 8,230 1 View

MRNA 3' sequence after in vitro transcription of linear DNA template?

Hi. I'm looking in to doing somein vitro transcription from a linear dsDNA template, or a linearised plasmid. When my T7 RNA pol runs along generating the mRNA, once its hits the 3' end of the...

30 May 2019 9,010 3 View

Can I use penicillin/streptomycin in a hygromycin selection process?

Hi all, I'm working on preparation of a stable cell line using hygromycin selection process. I'm using HEK293 cell line and 200 ug /mL of hygromycin-containing media. However, I found that all...

25 September 2018 2,693 3 View

Can i ues simethicone with surfactant?

Dear researchers. I have to coat tablets with simethicone oil. Since simethicone is too oily coating quality is very poor. So, i thinking of adding surfactant. (SLS or tween) The question...

16 September 2018 5,636 2 View

How vital is it to optimise total RNA Input for qPCR reactions?

How vital is it to optimize the RNA input for reverse transcription for subsequent qPCR reactions? The qPCR reaction mix protocol calls for 25ng of template. I've been using 100ng total RNA for...

05 March 2018 1,622 4 View

How can you fix cells without removing media?

I grow my neurons on glass surface. Their axons so delicate that I cannot remove the media entirely. When I refeed them, I remove half the media and replace it with fresh media containing double...

03 February 2018 1,918 3 View

Can I freeze a CoPi/ metal oxide in N2 before cutting it cross section for SEM?

Planning to do it this way, but cannot help worrying about possible changes that will happen to Co-Pi coating.

08 January 2018 5,440 2 View

Can I produce a significant amount of gypsum if I add sulfuric acid even before slaking of CaO occurs?

The paper attached claims to have produced around 600 g gypsum from 1 kg of egg shells. They did this by simultaneously adding CaO derived from calcination, water, and the sulfuric acid solution....

11 December 2017 7,452 1 View

Why can't we discriminate singlets using Width (Time of Flight) only?

It's my first experiment in flow cytometry. I'm attempting to exclude non-single cells. I'm wondering why it's not common practice to simply discriminate using pulse width (Time of Flight)? What...

28 November 2017 210 3 View

Can you perform fixation and immuno fluorescent staining in plastic 96 well plate tissue culture plates?

I grow my cells on both plastic Corning 96well culture plates for most experiments, but for immunoluorescence staining I'm growing them on glass or permanox chamber slides. One of the Chamber...

17 November 2017 8,208 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

What is the system of units used in the thermal conductivity by Spitzer and Harm?

23 February 2021 9,253 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Which thermal conductivity test recommended for Ag-Cu nanofluid?

I have prepared Ag-Cu alloy with polymer coating upon it.... how can I measure thermal conductivity of it ?

22 February 2021 5,915 3 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View