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Questions related to Annealing
Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...
01 January 2018 584 5 View
01 January 2018 5,627 7 View
Verification of empty pET28a+ identity before ligation of insert.
25 December 2017 6,279 3 View
Hi everyone, I need to calculate which fraction (percent) of a given oligo is annealed at given temperature (like 4°C, 10°C, 20 °C and 37°C ). I found the formula to calculate the Tm (50%...
22 December 2017 3,347 3 View
I'm trying to obtain a layer of Nickel Silicide in the 50:50 phase by diffusing a thin layer of evaporated Ni on a 100 oriented Si wafer. I have been able to obtain the supposedly metastable phase...
15 December 2017 2,972 3 View
System : Si/or Ge:substrate (200microns) HfO2: Deposited by ALD (10-15nm) Top metal electrode, TiN: In-situ ALD deposited (15nm) Therefore, final system is: TiN(15nm)/ HfO2(15nm)/Si(200microns)...
12 December 2017 10,029 2 View
12 December 2017 5,092 3 View
12 December 2017 1,209 3 View
I have a homework need to expand a stent directly by 3mm, and then anneal before crimping stent diameter by 3mm. I know that annealing can remove residual stresses in metal,but anneal temperature...
12 December 2017 2,672 0 View
Hello. I am dealing with sodium and lithium compounds, however it seems that a lot of sodium/lithium is evaporating during annealing process. Can you share your experience, how do you deal with...
12 December 2017 6,582 7 View
what is difference between pre-annealing temperature and annealing temperature
12 December 2017 6,098 2 View
12 December 2017 6,398 2 View
Sir/Madam I want to increase the CSL 3 fraction (i.e. mainly increase the twins ) just by any of the heat treatment technique (annealing ,tempering etc). The history of the sample is hot...
12 December 2017 8,460 3 View
12 December 2017 682 6 View
06 December 2017 10,234 3 View
One of the important factor on the membrane performance is heat treatment or thermal annealing. What is the effects of thermal annealing during membrane fabrication process on the membrane...
11 November 2017 7,839 2 View
Hi everyone, I have a couple of high GC content oligonucleotides that I need to anneal each other. One of those is 6-Fam labeled. For me it is important to have all the 6-Fam primer annealed....
11 November 2017 7,512 2 View
After annealing treatment of Austenitic stainless steel, what kind of reagent conrotion test the sigma- phases .
11 November 2017 8,334 0 View
11 November 2017 3,621 2 View
If in the cold rolled annealed steel the carbon content decreases from 0.52 to 0.40 how can I keep the hardness and springiness of the steel same as desired level by heat treatment?
10 November 2017 1,061 3 View
Dear respected fellows, Need your valuable suggestions for annealing process temperature . H13 tool steel with Aluminium chromium base coating used for die casting dies.
18 October 2017 4,852 2 View
Hi every one. I am looking for some suitable Laser annealing system in Tokyo area for Mg-doped p-type AlGaN annealing. I am aiming for p-type AlGaN UV LED. Do you have any idea? Thank you in advance
10 October 2017 5,257 2 View
I want to determine the heavy metal contamination of wood samples by ICP-MS. I have calcined the sample following the EN 14775(2010) method (each wood sample was heated at a rate of 4.5 °C/min. i....
10 October 2017 3,293 5 View
I followed this paper http://pubs.rsc.org/en/Content/ArticleLanding/2005/LC/b502809d#!divAbstract from MIT, but they used Pyrex glass. I do it the same but with B270 glass from Schott, I heated at...
10 October 2017 8,746 9 View