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Questions related from Wei Li
Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus and what else other methods that could help drug/virus to...
10 October 2018 4,925 4 View
(e-)books, web, database, ......... includes prokaryotic and eukaryotic plasmid vector electments
08 August 2018 1,229 0 View
I wish the books or references that could tell me why the morphology of these cells happen and what implications does such morphology will give us under designated conditions.
08 August 2018 5,017 0 View
why
08 August 2018 7,175 0 View
experimental videos tend to be more direct sometimes.
08 August 2018 3,575 1 View
Is it true, statements below? 1. Instead, the rAAV genome is typically processed into a double-stranded circular episome through double stranded synthesis. These episomes can concatemerize,...
08 August 2018 7,296 0 View
Are there any real cases in ways of bioimging or bioinformatics?
08 August 2018 5,661 0 View
Could you explain this from the level of Lac-associating gene and metabolic process?
06 June 2018 1,122 3 View
Except Addgene plasmid 101
05 May 2018 1,543 3 View
I know Addgene plasmid 101, only.
05 May 2018 6,521 3 View
I think it's because of internal reasons (e.g. temperature-sensitive ori) in plasmid itself and natural reasons (consume lots of resources in cells and probably digested by protease). In terms of...
05 May 2018 6,504 2 View
make recombinant protein amplify it in bacteria cell (E. coli) make sure the exact protein by PCR, electrophoresis, sequencing transfect (chemical methods) to mammal cells
04 April 2018 7,267 5 View
Could you pass me the original paper or tell me with your words?
04 April 2018 366 2 View
e.g. some sites in promoter will be blocked by the high level of CpG island when the cell develops into a certain stage. are there other factors that lead to the fact that the same gene expresses...
03 March 2018 2,057 0 View
High copy number is low expression amount of protein in E. coli cells When you do protein expression?
03 March 2018 315 3 View
Limitation: 1) target specific gene: 2) do epigenetic modification:
03 March 2018 3,984 6 View
the spot in microchip is quite small, so I guess fluorescence data in 96-well plates after being detected by Fluorescence Detector also need to be corrected using some ways of data handling
02 February 2018 7,176 2 View
please recommend some papers talking about Tat and Sec elements in plasmid vectors
01 January 2018 7,977 0 View
I wanna produce and publish a Cas9 gene therapy drug, but I don't know how to do, I lack of example and real case for this project. Could you recommend any real cases to me?
01 January 2018 1,551 0 View
it seems many senario that we have to/could do log transformation) e.g. make growth curve of bacteria, OD650 values could be transformed into corresponding log value, then plot growth curve. When...
01 January 2018 9,810 5 View
How to relatively accurately quantify the SDS-PAGE gel and DNA electrophoresis gel? Any methods?
01 January 2018 6,063 3 View
What's the benefits of such a basic step?
01 January 2018 7,586 7 View
e.g. I have a set of GFP-based membrane proteins, I detect the fluorescence intensity. I could say the more intense detected fluorescence, the more the amount of fusion protein. But what is the...
01 January 2018 2,024 5 View
Experiment: express a simple protein (e.g. membrane protein of E. coli) in E. coli in a way of fusing GFP and membrane protein. Linear? or exponential or else ??
01 January 2018 1,796 3 View
You could say many daily routines involving expression and purification of protein, but what experiments have to involve expression and purification of proteins? I wash obtain a general but...
01 January 2018 8,027 8 View
As shown in this picture, if the rate of protein sequence coverage is a result that the number of matched amino acids among MS/MS peptides is divided by the total number of amino acids in searched...
01 January 2018 1,867 1 View
add all numbers outside of the bracket in "matches"? like 1+1+1+1+1+.....
01 January 2018 1,665 4 View
Could you recommend any detailed reference books or papers introducing how to use proteomics-associated databases and then to analysise results for our long-term learning of proteomics...
01 January 2018 7,034 3 View
Could you post your papers or any papers showing the importance of membrane protein especially talking about two angles of industry and bioscience research.
01 January 2018 2,335 8 View
I have 2 slides. each of slides contains 2 channels scaned by Cy5 and Cy3 fluorescence excitaton. I have handled data by background effect correction by aligning the mean of each channel in 4...
12 December 2017 2,811 0 View
I am a English leaners, sometimes I need to use e-dictionary to look for technical terms or appropriate expression
10 October 2017 9,271 1 View
can you tell me the name of this softeware
10 October 2017 9,920 5 View
include ethic part, but not limited to this. mainly in operation and technology Is there any hindrance to this
10 October 2017 8,578 3 View
like the way that these pictures organize gene structure and genetic information. Can you show your snapshots, pictures, draft and other resources in your answer.
10 October 2017 2,865 3 View
Can I calculate it? if I can, how to calculate? if I lack of conditions? Or Why I cannot?
10 October 2017 8,619 2 View
Why develop conditional KO mouse including both tissue-specific and inducible mouse? A tissue-specific Knockout mouse defines a conditional biological model in which a target gene can be...
10 October 2017 6,165 2 View
A tissue-specific Knockout mouse defines a conditional biological model in which a target gene can be specifically inactivated in specific tissue(s); in all other tissues, the target gene exhibits...
10 October 2017 7,603 0 View
10 October 2017 6,593 0 View
1. Physiology is altered especially in 'immortalised' cell lines. 2. Cells pick up many mutations 3. Growth in nutrient rich media, often alters response to growth factors. 4. Environment cannot...
10 October 2017 1,298 0 View
Can you sort out those scientific questions requiring the use of cell culture?
10 October 2017 9,758 2 View
Why do human can cultivate eukaryotic cell? are there fundamental and underlying principles in cell culture?
10 October 2017 1,894 4 View
Can you list any differences of protein production by animal cell culture and microbial culture?
10 October 2017 6,644 4 View
A chemical question in fact.
10 October 2017 2,000 1 View
Core reasons is ？
10 October 2017 4,854 4 View
Why can it be argued that ES cell extraction accelerate the development of KO mouse model.
10 October 2017 8,856 2 View
is the statement that " As cells develop some genes are turned off permanently". correct? I pretty sure that there are other reason for this question if this answer is correct. Can you critically...
10 October 2017 8,273 3 View
develop knockut mouse
10 October 2017 3,370 3 View
which the stage/level of clinical trails are mouse models used in？ In the U.S., drug development has pre-clinic, first, second, third stage. Which the stage that required to use mouse models to...
10 October 2017 1,905 5 View
1. Explain what is meant by the term “knockout mouse”. 2. Describe how knockout mice are made overall strategy core method: traditional ways: TELENs, ZFNs, .... new ways:...
10 October 2017 2,820 1 View
