It is anthentic and reliable using ImageJ to quantify protein and DNA amount in SDS PAGE and eletrophoresis gel ?

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Sang Ho Lee

Hi Wei.

Image analysis of the bands is a relative measurement, a kind of semi-quantitation. It is reliable to compare in two and more bands. Protein bands are more close to real in relative terms than the amplified RT-PCR DNA bands, but not unamplified DNA fragments. This comparison is enough to estimate the amounts. This could be demonstrated by loading the known amounts of DNA and proteins together.

Regards.

Jordan R. Yaron

As Sang Ho Lee mentioned, densitometry is, at best, semi-quantitative.

Mostafa Haghpanah

dear Wei Li

I used Totallab software

http://totallab.com/