The sorts of scientific questions that require cell culture, giving examples of its use?

More Wei Li's questions See All

Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus?

Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus and what else other methods that could help drug/virus to...

09 October 2018 4,983 4 View

Why cannot we use prokaryotic cells (e.g. E. coli) or yeast or other low-cast cell lines to produce virus particles (e.g. rAAV or lentivirus)?

why

07 August 2018 7,215 0 View

Are there any books or references profiling morphology of various eukaryotic cells (especially mouse/human cells)?

I wish the books or references that could tell me why the morphology of these cells happen and what implications does such morphology will give us under designated conditions.

07 August 2018 5,044 0 View

Are there any websites demonstrating various biological experiments even in deepweb or darkweb (e.g. JoVE)?

experimental videos tend to be more direct sometimes.

07 August 2018 3,613 1 View

How to analyse advanced structure of a protein or protein complex in order to improve protein's some properties?

Are there any real cases in ways of bioimging or bioinformatics?

07 August 2018 5,703 0 View

Does rAAV have a potential risk to integrate a piece of DNA into host genome?

Is it true, statements below? 1. Instead, the rAAV genome is typically processed into a double-stranded circular episome through double stranded synthesis. These episomes can concatemerize,...

07 August 2018 7,320 0 View

Are there any resources introducing the interaction and mechanism of plasmid vector elements when you deliver plasmid into specific cells?

(e-)books, web, database, ......... includes prokaryotic and eukaryotic plasmid vector electments

07 August 2018 1,265 0 View

What molecular and genetic mechanism is that Lac+ E. coli shows red/pink color while Lac- E. coli shows white color on MacConkey plate?

Could you explain this from the level of Lac-associating gene and metabolic process?

05 June 2018 1,146 3 View

Are there any masterpiece teaching synthetic biology especially related with plasmids construction?

Except Addgene plasmid 101

04 May 2018 1,576 3 View

Are there any resources, tools, or references that can allow me to understand functions of various plasmid elements?

I know Addgene plasmid 101, only.

04 May 2018 6,556 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View