Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus?

Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus and what else other methods that could help drug/virus to...

09 October 2018 4,841 4 View

Why cannot we use prokaryotic cells (e.g. E. coli) or yeast or other low-cast cell lines to produce virus particles (e.g. rAAV or lentivirus)?

why

07 August 2018 7,082 0 View

Are there any books or references profiling morphology of various eukaryotic cells (especially mouse/human cells)?

I wish the books or references that could tell me why the morphology of these cells happen and what implications does such morphology will give us under designated conditions.

07 August 2018 4,921 0 View

Are there any websites demonstrating various biological experiments even in deepweb or darkweb (e.g. JoVE)?

experimental videos tend to be more direct sometimes.

07 August 2018 3,487 1 View

How to analyse advanced structure of a protein or protein complex in order to improve protein's some properties?

Are there any real cases in ways of bioimging or bioinformatics?

07 August 2018 5,561 0 View

Does rAAV have a potential risk to integrate a piece of DNA into host genome?

Is it true, statements below? 1. Instead, the rAAV genome is typically processed into a double-stranded circular episome through double stranded synthesis. These episomes can concatemerize,...

07 August 2018 7,208 0 View

Are there any resources introducing the interaction and mechanism of plasmid vector elements when you deliver plasmid into specific cells?

(e-)books, web, database, ......... includes prokaryotic and eukaryotic plasmid vector electments

07 August 2018 1,134 0 View

What molecular and genetic mechanism is that Lac+ E. coli shows red/pink color while Lac- E. coli shows white color on MacConkey plate?

Could you explain this from the level of Lac-associating gene and metabolic process?

05 June 2018 1,027 3 View

Are there any resources, tools, or references that can allow me to understand functions of various plasmid elements?

I know Addgene plasmid 101, only.

04 May 2018 6,418 3 View

Are there any masterpiece teaching synthetic biology especially related with plasmids construction?

Except Addgene plasmid 101

04 May 2018 1,450 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View