(e-)books, web, database, .........
includes prokaryotic and eukaryotic plasmid vector electments
Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus and what else other methods that could help drug/virus to...
09 October 2018 4,841 4 View
why
07 August 2018 7,082 0 View
I wish the books or references that could tell me why the morphology of these cells happen and what implications does such morphology will give us under designated conditions.
07 August 2018 4,921 0 View
experimental videos tend to be more direct sometimes.
07 August 2018 3,487 1 View
Are there any real cases in ways of bioimging or bioinformatics?
07 August 2018 5,561 0 View
Is it true, statements below? 1. Instead, the rAAV genome is typically processed into a double-stranded circular episome through double stranded synthesis. These episomes can concatemerize,...
07 August 2018 7,208 0 View
Could you explain this from the level of Lac-associating gene and metabolic process?
05 June 2018 1,027 3 View
I know Addgene plasmid 101, only.
04 May 2018 6,418 3 View
Except Addgene plasmid 101
04 May 2018 1,450 3 View
I think it's because of internal reasons (e.g. temperature-sensitive ori) in plasmid itself and natural reasons (consume lots of resources in cells and probably digested by protease). In terms of...
04 May 2018 6,423 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...
25 February 2021 1,011 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...
23 February 2021 5,167 3 View