Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus?

Why is intravenous injection a kind of systemic drug delivery (what areas cannot this injection method reach when we deliever drug/virus and what else other methods that could help drug/virus to...

09 October 2018 4,841 4 View

Why cannot we use prokaryotic cells (e.g. E. coli) or yeast or other low-cast cell lines to produce virus particles (e.g. rAAV or lentivirus)?

why

07 August 2018 7,082 0 View

Are there any books or references profiling morphology of various eukaryotic cells (especially mouse/human cells)?

I wish the books or references that could tell me why the morphology of these cells happen and what implications does such morphology will give us under designated conditions.

07 August 2018 4,921 0 View

Are there any websites demonstrating various biological experiments even in deepweb or darkweb (e.g. JoVE)?

experimental videos tend to be more direct sometimes.

07 August 2018 3,487 1 View

How to analyse advanced structure of a protein or protein complex in order to improve protein's some properties?

Are there any real cases in ways of bioimging or bioinformatics?

07 August 2018 5,561 0 View

Does rAAV have a potential risk to integrate a piece of DNA into host genome?

Is it true, statements below? 1. Instead, the rAAV genome is typically processed into a double-stranded circular episome through double stranded synthesis. These episomes can concatemerize,...

07 August 2018 7,208 0 View

Are there any resources introducing the interaction and mechanism of plasmid vector elements when you deliver plasmid into specific cells?

(e-)books, web, database, ......... includes prokaryotic and eukaryotic plasmid vector electments

07 August 2018 1,134 0 View

What molecular and genetic mechanism is that Lac+ E. coli shows red/pink color while Lac- E. coli shows white color on MacConkey plate?

Could you explain this from the level of Lac-associating gene and metabolic process?

05 June 2018 1,027 3 View

Are there any resources, tools, or references that can allow me to understand functions of various plasmid elements?

I know Addgene plasmid 101, only.

04 May 2018 6,418 3 View

Are there any masterpiece teaching synthetic biology especially related with plasmids construction?

Except Addgene plasmid 101

04 May 2018 1,450 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View