You could say many daily routines involving expression and purification of protein, but what experiments have to involve expression and purification of proteins?
I wash obtain a general but essential statement that answers from different research or aspects.
I wanna modulize and industrilize basic experiment in lab using robots and mechines. —— my ultimate intention.
I can only roughly imagine the intention of your question but can give the following short answer:
It's important to know the levels of proteins within cells and also their properties as they are the main tools and machines driving all cell processes, such as catalyzing chemical reactions, transporting substances or controlling biochemical parameters. Therefore, processes of their 'expression' or of their own regulation such as by posttranslational modifications are substantial for understanding their functions in the organism and the function of a cell or organ.
In addition to what Prof.Roos has mentioned, I may add that you need to
i) purify a protein to comprehend its structure, function, kinetics etc. uncontaminated by other proteins/compounds
ii) express the purified protein to generate it in larger amounts for studying the aforesaid parameters and/or if you are trying to produce sufficient amounts of the purified protein for a particular purpose.
You are serious. I thought this question was a flame bait or from a troll.
Have you ever worked with DNA?
All those restriction enzymes, polymerases, ligases, etc. are purified proteins. Without those purified enzymes, there would be no genetic engineering.
Making specific antibodies against specific proteins for research is dependent on using purified proteins as immunogens.
Immunotherapies against cancer? Antibodies are made against purified proteins. Yes and the antibodies purified as well.
Do these examples answer your question? If not, I can give you examples of purified enzymes/proteins being vital for medicinal chemistry, food chemistry, environmental sciences, toxicology, etc.
Name any branch of life science and I can give you examples of their reliance on purified proteins.
The three-dimensional structures of proteins can only be determined by first purifying the proteins and then performing x-ray crystallography, multidimensional NMR, and/or cryo-electron microscopy.
There are already several structural genomics groups that automate the process of expressing and purifying proteins, setting up crystallization experiments, and collecting x-ray diffraction data. You would not be a pioneer in this area.
In drug discovery, structure-guided drug design is often practiced. The structure of the protein with bound inhibitors is used, in combination with biochemical data about the potency of the inhibitor, in an iterative fashion, to develop ideas for improved inhibitors.
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