Are the fluorescence data form Fluorescence Detector in 96-well plates required to correct variation using normalizaiton or backgrounnd correction?

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Adam B Shapiro

It is always a good idea to measure the background signal from another well in which the fluorescent substance has been omitted. This background can then be subtracted.

If you are trying to measure the fluorescence from a small solid object in the well, you should use a plate reader that is capable of sampling from multiple positions within the same well, to make sure the object is detected.

Samir Begaa

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