Why will artificial plasmids inside cells lose over time/generation?

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Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

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Kou Hayakawa

I would like to express my opinion.

Bacteria (Lactobacillus casei (Shirota)) has c.a. 12% bacteriophage (our unpublished observation).

HepG2 has 6.6% virus, and Hc has 4.7% virus, 10.0% bacteria and 3.2% plant (please see file; HepG2 fucoidan).

Therefore, the severe battle between aborigines and immigrant virus to get the hegemony seems usually to be occurred inside the host cells.

Dominique Liger

Hi there,

Plasmid loss occurs only when it is not essential for cell survival (ie. when there is no selection pressure).