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Questions related from Hafiz Muhammad Rizwan
i would like to know that i have isolated about 30 different kind of isolates from fruit peel at harvesting stage..and have pure colonies and i want to do Identification, so i have finished...
01 January 2020 6,706 7 View
I have 20-25 different types of fungal isolates, ( isolated from fruit peel after harvesting) and now I would like to do identification, 1- some grow faster ( to make colony) and some grow, so...
01 January 2020 3,450 4 View
Dear Researchers, 1- I am working on RNA seq, and now I have transcriptome data and I have the different genes of interest from different resources, but I am looking for their full-length CDs...
01 January 2020 2,852 3 View
Deaer Researchers, I have isolated 20 to 30 fungal isolates from fruit and now have pure colonies . so i want to identify them on morphologically which fungi are those, some isolates...
12 December 2019 2,809 5 View
which software is easy and best to use for SEM, scanning electron microscopy images analysis .. such as for stomata area. trichome length. trichome tickness. fruit peel different layers. etc
12 December 2019 8,017 6 View
Dear Researchers, I did Transcriptome of my two Fruit varieties at developmental, ripening and harvesting stage for the comparison of their differential gene expression profile, such as Sugar...
12 December 2019 8,171 3 View
I am working on fruit cuticle wax biosynthesis and did the transcriptome and now I have collected many genes from different sources such as from articles and online etc, for example, CER1, CER3,...
12 December 2019 528 11 View
I have high concentration RNA and good quality as attached images, but when run the Gel then no clear bands, and i have tried different methods and used other RNA sample to make sure...
09 September 2019 2,861 3 View
dear researcher, i have extracted RNA having 400-600 ng/ul concentration, A260/A280 is 1.98-2.00, but when i run gel there is no clear bands, so i have tried different ways to get...
09 September 2019 8,611 1 View
I have extracted RNA by usung Trizol method from fruit peel, and during nanodrop biospectrometer facing this error as in image. so need your suggestion . thanks
09 September 2019 8,971 4 View
Dear Researchers, I did the qPCR, 20ul, (10 ul TB gree, primer 0.4, template 2ul, Water 7.2), steps, by using TAKARA, I got the following results, as you can see that with 1X, 3X, and 5X...
09 September 2019 644 7 View
Dear Researchers, i have extracted the RNA ,having high concentration and good quality...but when run the gel...so the bands not clear...and i am using 90 V , 400 mAP. for 20...
09 September 2019 4,489 8 View
Dear Researchers, I have extracted the RNA by using the Qiangen Kit and also Tiangen Kit , by using passion fruit Peel at ripening stages, But the concentration is about 20 -50 ng/ul ....
09 September 2019 7,222 2 View
i have RNA Seq Transcriptome data from company, but now i dont know how to analysis step by step.. can someoen share link about step by step guidence to analysis the transcriptomic data .?...
09 September 2019 777 8 View
i have RNA transcriptomic data and i would like to select a pathway for further analysis, but in transcriptomic ,have many pathways, so on which basis, and how to select a specific...
09 September 2019 7,692 3 View
I have extracted RNA by Trizol method, and got good Nano drop results, (as can see in images), but when I run the Gel 1%, at 90-120 V, for 20-30 minutes, then got no bands, ( I have many times,...
09 September 2019 9,267 7 View
Dear Researcher I am working on passion fruit peel at ripening stages, and want to do qPCR but facing problem in getting good quality RNA from fruit peel, I have tried to extract the RNA...
09 September 2019 8,988 3 View
Dear Researchers, I did the Gas chromatography mass spectrometery ( GC-MS) by using Agilent instrument, and now the Agilent software is not working in my computer, so any other easily...
09 September 2019 7,209 15 View
Dear Researchers, I have extracted RNA from passion fruit peel from fruit setting to ripening stages, (1- 10 developmental stages), by using Qiagen Mini plant Kit, from 1-5 stages the...
08 August 2019 4,437 0 View
Dear Researchers\ I did scanning electron microscopy (SEM) of the fruit peel and founded different structure during SEM, from which some i know like trichomes, lenticles/ stomata, wax crystles,...
08 August 2019 4,193 4 View
Dear Resrearchers, I am doing Gas Chrometography data analysis by using Aligent Qualitative Analysis B.07.00 and NIST 2.00 for cuticle wax compounds identification, (biochemistry) I would like...
07 July 2019 8,537 5 View
Dear researchers, i would like to make a research on "high quality organic fertilizer production from biogas extracts" looking forward to your sugessions
06 June 2019 4,905 15 View
Dear researchers, If i would like to know the Retention Time of a compound use as internal standard so how can i know about its retention time?
06 June 2019 6,633 4 View
Dear researchers Can anyone share this software with me"Agilent MassHunter GCMS Data Acquisition" (64 bit) Thanks in advance
05 May 2019 467 2 View
Dear Researcher, I am working on passion fruit juice, I have extracted fruit juice and kept in 4 oC, and i will measure sugar, acids and other nutritional contents from fruit juice...
01 January 2019 555 2 View
Hello Researcher, I am working on Passion fruit and i need a protocol for sample preparation for sugar acids in passion fruit juice by HPLC, waiting for your kind suggestions and references
01 January 2019 5,475 10 View
Hello Everyone, extracted the fruit juice from a fresh harvested ripened fruit bu due to the shortage of time we kept our fruit sample in refrigerator for further study ( pH, sugar, acidic...
12 December 2018 5,023 9 View
Hello Researchers, I am working on passion fruit, i would like to know that how can extract the cutical wax from passion fruit ? i have read articles related to different fruits but in...
12 December 2018 643 0 View
Scanning electron microscopy of peel surface of fruit? Hello ever oneI have done Scanning Electron Microscopy ( SEM) for fruit surface (horizontally) at fully ripened stage. in which i have seen...
12 December 2018 5,438 0 View
Hello everyone, I would like to know, anyone is working on somatic embryogenesis/ callus induction / organogenesis ? i need some suggestions and some articles related to the reasons behind...
02 February 2018 8,249 12 View
Hi, I want to know explanation about the embryogenic and non-embryogenic callus in okra, so how can differentiate that is this embryogenic or non embryogenic callus? about color ,...
01 January 2018 6,878 1 View
I am working on Okra tissue culture but i am facing problem about okra seed germination , for seed treatment i am using HgCl2 0.1% for 3 minutes and after washing with dd water use 75 %...
11 November 2017 1,755 6 View
Hello ever one I have done Scanning Electron Microscopy ( SEM) for fruit surface (horizontally) at fully ripened stage. in which i have seen the following images (A) Hole like structure...
01 January 1970 8,647 0 View
