How to find a Gene full length cds or Seq having gene name but not having gene ID?

Dear Researchers, 1- I am working on RNA seq, and now I have transcriptome data and I have the different genes of interest from different resources, but I am looking for their full-length CDs...

31 December 2019 2,714 3 View

What is the best method for Fungi identification ? RNA Seq? DNA Seq? and whats the Protocol?

I have 20-25 different types of fungal isolates, ( isolated from fruit peel after harvesting) and now I would like to do identification, 1- some grow faster ( to make colony) and some grow, so...

31 December 2019 3,336 4 View

HOW to prepare fungal isolate sample for DNA extraction ?

i would like to know that i have isolated about 30 different kind of isolates from fruit peel at harvesting stage..and have pure colonies and i want to do Identification, so i have finished...

31 December 2019 6,518 7 View

How can identify a fungal isolate with out RNA or ITS Seq?

Deaer Researchers, I have isolated 20 to 30 fungal isolates from fruit and now have pure colonies . so i want to identify them on morphologically which fungi are those, some isolates...

11 December 2019 2,657 5 View

Is there any specific method or procedure for Transcriptome data analysis?

Dear Researchers, I did Transcriptome of my two Fruit varieties at developmental, ripening and harvesting stage for the comparison of their differential gene expression profile, such as Sugar...

11 December 2019 8,043 3 View

Which is the best software for microscopy image data analysis, such as stomata size, cuticle wax layer thickness ?

which software is easy and best to use for SEM, scanning electron microscopy images analysis .. such as for stomata area. trichome length. trichome tickness. fruit peel different layers. etc

11 December 2019 7,901 6 View

How to make sure that either that gene is identified gene or not?

I am working on fruit cuticle wax biosynthesis and did the transcriptome and now I have collected many genes from different sources such as from articles and online etc, for example, CER1, CER3,...

11 December 2019 408 11 View

Which is the easiest software for GC-MS data analysis?

Dear Researchers, I did the Gas chromatography mass spectrometery ( GC-MS) by using Agilent instrument, and now the Agilent software is not working in my computer, so any other easily...

08 September 2019 7,082 15 View

Having high concentration and quality of RNA , is necessary to get clear bands for cDNA and qPCR?

dear researcher, i have extracted RNA having 400-600 ng/ul concentration, A260/A280 is 1.98-2.00, but when i run gel there is no clear bands, so i have tried different ways to get...

08 September 2019 8,479 1 View

Are low concentration RNA useful for cDNA and qPCR ?

Dear Researchers, I have extracted the RNA by using the Qiangen Kit and also Tiangen Kit , by using passion fruit Peel at ripening stages, But the concentration is about 20 -50 ng/ul ....

08 September 2019 7,054 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View