How to find a Gene full length cds or Seq having gene name but not having gene ID?

Dear Researchers, 1- I am working on RNA seq, and now I have transcriptome data and I have the different genes of interest from different resources, but I am looking for their full-length CDs...

31 December 2019 2,714 3 View

What is the best method for Fungi identification ? RNA Seq? DNA Seq? and whats the Protocol?

I have 20-25 different types of fungal isolates, ( isolated from fruit peel after harvesting) and now I would like to do identification, 1- some grow faster ( to make colony) and some grow, so...

31 December 2019 3,336 4 View

HOW to prepare fungal isolate sample for DNA extraction ?

i would like to know that i have isolated about 30 different kind of isolates from fruit peel at harvesting stage..and have pure colonies and i want to do Identification, so i have finished...

31 December 2019 6,518 7 View

How can identify a fungal isolate with out RNA or ITS Seq?

Deaer Researchers, I have isolated 20 to 30 fungal isolates from fruit and now have pure colonies . so i want to identify them on morphologically which fungi are those, some isolates...

11 December 2019 2,657 5 View

Is there any specific method or procedure for Transcriptome data analysis?

Dear Researchers, I did Transcriptome of my two Fruit varieties at developmental, ripening and harvesting stage for the comparison of their differential gene expression profile, such as Sugar...

11 December 2019 8,043 3 View

Which is the best software for microscopy image data analysis, such as stomata size, cuticle wax layer thickness ?

which software is easy and best to use for SEM, scanning electron microscopy images analysis .. such as for stomata area. trichome length. trichome tickness. fruit peel different layers. etc

11 December 2019 7,901 6 View

How to make sure that either that gene is identified gene or not?

I am working on fruit cuticle wax biosynthesis and did the transcriptome and now I have collected many genes from different sources such as from articles and online etc, for example, CER1, CER3,...

11 December 2019 408 11 View

Which is the easiest software for GC-MS data analysis?

Dear Researchers, I did the Gas chromatography mass spectrometery ( GC-MS) by using Agilent instrument, and now the Agilent software is not working in my computer, so any other easily...

08 September 2019 7,082 15 View

Having high concentration and quality of RNA , is necessary to get clear bands for cDNA and qPCR?

dear researcher, i have extracted RNA having 400-600 ng/ul concentration, A260/A280 is 1.98-2.00, but when i run gel there is no clear bands, so i have tried different ways to get...

08 September 2019 8,479 1 View

Are low concentration RNA useful for cDNA and qPCR ?

Dear Researchers, I have extracted the RNA by using the Qiangen Kit and also Tiangen Kit , by using passion fruit Peel at ripening stages, But the concentration is about 20 -50 ng/ul ....

08 September 2019 7,054 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View