Hello Researcher,
I am working on Passion fruit and i need a protocol for sample preparation for sugar acids in passion fruit juice by HPLC,
waiting for your kind suggestions and references
Maybe the following publications are usefull for you
https://www.agilent.com/cs/library/applications/5990-8801EN%20Hi-Plex%20Compendium.pdf
Article Quantification of sugars and organic acids in tomato fruits
Adapt the ICUMSA (International Commission of Uniform Methods of Sugar Analysis) method.
Bruce Neagle thanks, can you please explain ? i not understand
There are HPLC methods for Sugar Analysis. They are very common. I would just homogenize the sample in water. Store it in the refrigerator in order to prevent yeast fermentation of the mash. Filter a sample (0.22 um), and inject into the HPLC.
1. Method 1 is a Water's Sugarpak column (or equivalent like Rezex or Hi-Plex) using a 85 Celsius water mobile phase and a 'Refractive Index Detector (RID)'.
2. Method 2 is a Biorad Aminex column (or equivalent) and a PDA/DAD set at 210 nm.
3. Other detectors like PAD....
Bruce Neagle Thanks a lot sir ,
Sir , do you know the GC-MS condition for cuticle wax composition compounds?
Nope! 1st you have to be able to dissolve the wax in a non-polar solvent like Hexane. Try an internet search on Google Scholar.
Bruce Neagle Dear sir, i have extracted the wax in chloroform chemical ,
Now i would like to do GC-MS , but the problem is that according to article the temperature for column should be 320C by GC MS but in our lab GC MS can only support up to 280 C due to which i have not got a very good results, so whats your suggestions??
I have none! Each zone has its own temperature maximum manually inserted. The 280 Celsius maximum could be due to the type of column you are using (Wax I presume).
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