Hight concentration with good quality but no clear bands?

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Miguel Fernández-Niño

Hi.

How much ng are you running? usually you should run 100ng.

Are you avoiding overheating of the gel?

Maybe try 60v in a cold room, less than 1 hour.

Are you treating your thank and combs with DEPC?

Hafiz Muhammad Rizwan

Miguel Fernández-Niño running 1000ng

yes, by using ice tray

thanks and comb were not treated

Md Abdur Rahim Thanks i will