Dear Researchers,
I have extracted RNA from passion fruit peel from fruit setting to ripening stages, (1- 10 developmental stages), by using Qiagen Mini plant Kit, from 1-5 stages the concentration is about 200-400 ng/ul with A260/280 = 1.98-2.1 , But in later stages 6-10 stages the RNA concentration very low 40-60 ng/ul with A260/280 = 1.98-2.1,
I have used 100-250 mg ( powder peel samples) for RNA extraction but the results still same, (The fruit samples for fresh taken by me and after drying in liquid nitrogen stored at -80 , and now extracting RNA after 1 month)
Can anyone help me, how can improve to get high quality RNA , or any other method or modification
Thanks
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