You need to run your gel longer. I recommend TBE buffer, 1% agarose. Formaldehyde will help with the secondary structure if you are running a denaturing gel. For what purpose are you running an RNA gel?

Also check OD A260/A230 ratio, it should be very close to 2,0. Ratio less than 2.0 indicates contamination with chaotropic salts or phenol or protein in the RNA solution.

260/280 ratio absorbance is also contributed by free nucleotides and RNA might not be intact.

Total RNA run on a denaturing agarose gel will show two distinct ribosomal peaks corresponding to either 18S and 28S for eukaryotic RNA. Intact RNA with a 28S:18S rRNA has ratio of approximately 2:1 (Look into intensity of band). If you are having smear or no band means your RNA is degraded.

You can also check RNA integrity by 2100 Bioanalyzer.

http://w1.iata.csic.es/IATA/usct/geno/Doc/Bibliografia/Quality%20assessment%20of%20total%20RNA.pdf

https://www.promega.com/resources/pubhub/methods-of-rna-quality-assessment/

The only bands you should expect to see are the ribosomal RNA bands as mentioned above. I think I can see them in some of the lanes of your gel, but yes there does appear to be a gel problem. You are not going to see distinct bands of mRNA because it is a population of thousands or millions of different molecules and should be a smear.

Craig Silver I will do qPCR

So if having high concentration and quality, but there is no clear bands , so can do direct cDNA and qPCR?

is necessary to get clear bands?

Bhairavi Vajaria Thanks for nice sharing , i will try

Nope, not necessary to get clear bands. You can proceed with cDNA and qPCR, based upon the spectrometer readings, good ratios and concentrations.

Best of luck!

Craig Silver how can make sure that my cDNA has been synthesized for qPCR?

You can just test it by qPCR. There is no good way to check this, if you run it on a gel it will be inconclusive and smeary due to the amplicons (this is normal) and spectrometry (nanodrop or qubit) is not too conclusive for this sort of determination. I would just test a house keeping gene (gapdh, beta-actin). Once you have your cDNA, you can just do an rt-PCR (use your cDNA as template, do a normal endpoint PCR, and run on a gel), and see if you see a band of expected size. Then proceed with qPCR (which uses more expensive reagents) if you are satisfied. Most people skip that step and go from cDNA to qPCR directly.

Best of luck!