Neede a Gas Chromatography–Mass Spectrometry GC/MS data analysis software?

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How to find a Gene full length cds or Seq having gene name but not having gene ID?

Dear Researchers, 1- I am working on RNA seq, and now I have transcriptome data and I have the different genes of interest from different resources, but I am looking for their full-length CDs...

31 December 2019 2,714 3 View

What is the best method for Fungi identification ? RNA Seq? DNA Seq? and whats the Protocol?

I have 20-25 different types of fungal isolates, ( isolated from fruit peel after harvesting) and now I would like to do identification, 1- some grow faster ( to make colony) and some grow, so...

31 December 2019 3,336 4 View

HOW to prepare fungal isolate sample for DNA extraction ?

i would like to know that i have isolated about 30 different kind of isolates from fruit peel at harvesting stage..and have pure colonies and i want to do Identification, so i have finished...

31 December 2019 6,518 7 View

How can identify a fungal isolate with out RNA or ITS Seq?

Deaer Researchers, I have isolated 20 to 30 fungal isolates from fruit and now have pure colonies . so i want to identify them on morphologically which fungi are those, some isolates...

11 December 2019 2,657 5 View

Is there any specific method or procedure for Transcriptome data analysis?

Dear Researchers, I did Transcriptome of my two Fruit varieties at developmental, ripening and harvesting stage for the comparison of their differential gene expression profile, such as Sugar...

11 December 2019 8,043 3 View

Which is the best software for microscopy image data analysis, such as stomata size, cuticle wax layer thickness ?

which software is easy and best to use for SEM, scanning electron microscopy images analysis .. such as for stomata area. trichome length. trichome tickness. fruit peel different layers. etc

11 December 2019 7,901 6 View

How to make sure that either that gene is identified gene or not?

I am working on fruit cuticle wax biosynthesis and did the transcriptome and now I have collected many genes from different sources such as from articles and online etc, for example, CER1, CER3,...

11 December 2019 408 11 View

Which is the easiest software for GC-MS data analysis?

Dear Researchers, I did the Gas chromatography mass spectrometery ( GC-MS) by using Agilent instrument, and now the Agilent software is not working in my computer, so any other easily...

08 September 2019 7,082 15 View

Having high concentration and quality of RNA , is necessary to get clear bands for cDNA and qPCR?

dear researcher, i have extracted RNA having 400-600 ng/ul concentration, A260/A280 is 1.98-2.00, but when i run gel there is no clear bands, so i have tried different ways to get...

08 September 2019 8,479 1 View

Are low concentration RNA useful for cDNA and qPCR ?

Dear Researchers, I have extracted the RNA by using the Qiangen Kit and also Tiangen Kit , by using passion fruit Peel at ripening stages, But the concentration is about 20 -50 ng/ul ....

08 September 2019 7,054 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View