Hi Hafiz,

The good news is that you plenty of RNA. The bad news is that your A260/280 ratio is fairly low, which often means you have some sort of protein contamination. Aromatic proteins have a strong UV absorbance at 280nm. Normally you would like to see a A260/280 ratio ~2 for pure RNA.

The abnormally high A260/230 ratio is often due to an incorrect blanking solution.

Here is a ThermoFisher resource:

https://assets.thermofisher.com/TFS-Assets/CAD/Product-Bulletins/TN52646-E-0215M-NucleicAcid.pdf

Best,

Max

As Max already said, your RNA seems to be "contaminated" with quite a lot of protein. Did you somehow purify your RNA already prior to do the measurements? You could try to measure with a 10-fold dilution (or even more) sample of your RNA with distilled and nuclease-free water, to see, whether the values can then be displayed.

Hello Hafiz

To solve the warning I would recommend to dilute your sample with ddH2O, 1 to 5, then start measuring and see what happen. Perhaps the source/s of error might be absorption at 230 or other wavelengths, first you need to observe by diluting.

Best regards

Hi!

I agree with Ali Javadmanesh , dilute the sample!

Dilute your sample 1:10 and read again. This will change the RNA concentration reading, off course, and the ratios 260/280; 260/230 (because the equipament will measure better, inside the nanodrop dynamic range ).

All equipaments have limit of detection and limits of operation. If you operate out of range, the equipament cannot measure correctly; this is what your mesage error is telling you. The mesage is " some measured abs values are too high and cannot be displayed".

Best,

Jacó