26 Questions 13 Answers 0 Followers
Questions related from Ameena Stellixir
Please suggest some standard drug and its IC50 concentration which is applicable for CHO cell line.
15 November 2023 7,564 0 View
As part of angiogenesis study, we did CAM assay with two replicates. The data we needed is of vascularization degree. But, the values in 0th and 24th hour first replicate is not similar to the...
31 October 2023 7,213 0 View
We are planning to do ELISA/ spectroscopy-based study. How to make it for time and dose-dependent assay, like OD values should be taken for every 20secs for 20 mins? Hence, how to calculate Vmax...
19 October 2023 9,202 0 View
I would like to read on papers related to CHO-K1 karyotyping, but could not able to find any. Can anyone recommend papers related to this.
11 August 2023 4,821 1 View
I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...
03 July 2023 9,293 1 View
03 July 2023 6,452 0 View
03 July 2023 4,021 0 View
Currently received this cell line L5178Y for some cell works. The prescribed media for the cell line was DMEM, but as it a suspension cell, the cells are maintained in RPMI media also. At the...
27 May 2023 2,199 0 View
I have to check the cytotoxicity of a drug using MTT assay. The drug is soluble in 90% alcohol, not in DMSO. Is 90% alcohol toxic to cells?
13 May 2023 7,740 3 View
I have done DAPI staining in untreated and drug-treated EA carcinoma cells using 96-well plate. The procedure included only pbs washes, fixation with paraformaldehyde and incubation of each wells...
04 May 2023 9,034 3 View
Planning to do DAPI staining using fluorescence microscopy. Thought of seeding the cells in 6-well plate. What all things should be considered while doing the assay? Does anyone have protocol for...
27 April 2023 3,846 3 View
I have to CAM assay for some samples. I'm planning to conduct the assay in eggs only, but is it better to conduct it in petriplates? While adding drugs (on eggs), whether we should inject it to...
26 April 2023 7,802 0 View
I would like to know the IC50 value of the drugs, cisplatin, camptothecin, quercetin, quinancrine, crizotinib, against the HT29 and EA carcinoma cell lines for 24, 48 and 72 hours of incubation.
18 April 2023 2,883 2 View
When silver nanoparticle used as test compound for cytotoxicity studies (MTT), the results showed like the compound is toxic upto a concentration, then onwards increased cell viability with...
14 April 2023 1,156 1 View
For an in-vitro scratch assay to be conducted using a collagen sheet, what is the solubility of the collagen sheet and how can we prepare the working concentration of the sheet (i.e., 0.025%)?
05 April 2023 789 0 View
Anyone know IC50 of cyclophosphamide against T24 cell line?
03 April 2023 3,578 6 View
I have to some microtitre-plate based antioxidant assays such as ORAC assay, DPPH and FRAP. If anyone has good working protocol, please do kindly share.
24 March 2023 4,826 1 View
I'm currently searching for comet assay kit, which can give almost accurate results and should be cheap of cost. Can anyone recommend any kits regarding this that you have worked with?
20 March 2023 1,397 2 View
The drug meant to be used is a gel. For dermal cytotoxicity study, have to conduct MTT assay in HaCaT cells using this gel with an incubation of 24hours. So, as standard group shouldn't we use an...
08 March 2023 9,485 0 View
My team is supposed to do angiogenesis signaling pathway based on ELISA method using VEGF marker. But we couldn't find any protocol other than kit method. If anyone has the protocol, please do...
28 February 2023 8,263 0 View
How to set scale bar in the image using image j? What to mention against "known distance" in the setting scale menu?
17 February 2023 8,539 2 View
For preparation of lysis solution for comet assay we needed tris base. But we found tris buffer on our stocked. When searched on internet both were having same formula.
16 February 2023 873 1 View
We did comet assay based on joves protocol. Under fluorescence microscope, cells were visible but without tail. The drugs showed toxicity when grown in 6-well plate. Lysis was done for overnight...
15 February 2023 1,885 9 View
I did prepare 1litre of comet assay lysis solution by adding sodium lauryl sarcosinate. For the assay used only 100 or 150ml and kept the remaining at 4 degree celsius for future use. After 2...
14 February 2023 2,322 4 View
I conducted mdamb 231 cells scratch assay in 6-well plate. Tried taking images under microscope (10X) for 0th hour right after the assay, but couldn't able to take properly. Lookslike some of the...
13 February 2023 7,581 3 View
I did a MTT assay for a plant extract against L929 cells. But in readings, the cell viability percentage was increased more than the untreated cells, with increasing concentration.
01 February 2023 2,608 1 View
