For preparation of lysis solution for comet assay we needed tris base. But we found tris buffer on our stocked. When searched on internet both were having same formula.
Hello Ameena Stellixir
Tris buffers comprise buffers based on the chemical compound Tris (tris(hydroxymethyl)aminomethane) while the term tris refers to any compound containing the tris(hydroxymethyl)aminomethane group. Usually, tris(hydroxymethyl)aminomethane itself is named as “tris base”, but there can be some other groups associated with this group. For example, tris HCl contains tris(hydroxymethyl)aminomethane in association with an HCl molecule.
Tris buffers can be made by using Tris Base and titrating with a hydrochloric acid solution to the desired pH value.
Determining whether to use tris HCl or tris base depends on the protocol you’re working with. When you need to adjust the pH of your solution, you can use the conjugate acidic or basic component. For example, if using a protocol calling for tris HCl, you can adjust the pH using tris base or if you need to lower the pH of your tris buffer, choose tris HCl and if, however, you need to increase the pH of your buffer, use tris base.
For preparing lysis solution for comet assay, I would recommend that you use tris base and not tris buffer as tris base would be the right choice.
Best.
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