The IC50 values of standard drugs against HT29 and EA carcinoma cell lines for 24, 48, and 72 hours are not available. Different studies measure IC50 values at different time points and concentrations, making it difficult to provide a standardized list of values.

You can get values suitable for comparisons you want to make with new conditions. Just make your plating in a very reproducible way, treat, and collect by methanol fixation. With Giemsa stain you can integrate the intensity of stain, which is proportional to the number of cells. Or if you plate at very low densities, you may be able to count colonies by 72 hours.