My team is supposed to do angiogenesis signaling pathway based on ELISA method using VEGF marker. But we couldn't find any protocol other than kit method. If anyone has the protocol, please do kindly share.
Please suggest some standard drug and its IC50 concentration which is applicable for CHO cell line.
14 November 2023 7,586 0 View
As part of angiogenesis study, we did CAM assay with two replicates. The data we needed is of vascularization degree. But, the values in 0th and 24th hour first replicate is not similar to the...
30 October 2023 7,235 0 View
We are planning to do ELISA/ spectroscopy-based study. How to make it for time and dose-dependent assay, like OD values should be taken for every 20secs for 20 mins? Hence, how to calculate Vmax...
18 October 2023 9,335 0 View
I would like to read on papers related to CHO-K1 karyotyping, but could not able to find any. Can anyone recommend papers related to this.
10 August 2023 4,838 1 View
I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...
02 July 2023 9,319 1 View
02 July 2023 6,467 0 View
02 July 2023 4,050 0 View
Currently received this cell line L5178Y for some cell works. The prescribed media for the cell line was DMEM, but as it a suspension cell, the cells are maintained in RPMI media also. At the...
26 May 2023 2,216 0 View
I have to check the cytotoxicity of a drug using MTT assay. The drug is soluble in 90% alcohol, not in DMSO. Is 90% alcohol toxic to cells?
12 May 2023 7,759 3 View
I have done DAPI staining in untreated and drug-treated EA carcinoma cells using 96-well plate. The procedure included only pbs washes, fixation with paraformaldehyde and incubation of each wells...
03 May 2023 9,064 3 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
The application of estrogen to wounds reduced inflammatory responses and promoted angiogenesis and woundcontraction more than the two other standard treatment methods.
09 July 2024 8,236 0 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
Hello, From my (naive) understanding, there are several target genes of the canonical Wnt signaling pathway that serve as reliable/validated transcriptional readouts of pathway activity. These...
18 June 2024 4,699 0 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View