Can anyone suggest working protocols for some antioxidant assays (ORAC, DPPH & FRAP?

24 March 2023 1 5K Report

I have to some microtitre-plate based antioxidant assays such as ORAC assay, DPPH and FRAP. If anyone has good working protocol, please do kindly share.

Khushboo Bhardwaj

Kindly follow the protocols as follow:

DPPH assay by Blois, 1958

FRAP assay by Benzie and Strain 1996

Badges
Science topic

More Ameena Stellixir's questions See All

Is there any standard drug that can be used as positive control for CHO cell line based MTT assay?

Please suggest some standard drug and its IC50 concentration which is applicable for CHO cell line.

14 November 2023 7,586 0 View

CAM assay replicate values should be same or not?

As part of angiogenesis study, we did CAM assay with two replicates. The data we needed is of vascularization degree. But, the values in 0th and 24th hour first replicate is not similar to the...

30 October 2023 7,235 0 View

Protocol for ELISA and analysis of Vmax (enzyme kinetics)?

We are planning to do ELISA/ spectroscopy-based study. How to make it for time and dose-dependent assay, like OD values should be taken for every 20secs for 20 mins? Hence, how to calculate Vmax...

18 October 2023 9,335 0 View

Can anyone suggest articles related to CHOK1 karyotyping or idiogram?

I would like to read on papers related to CHO-K1 karyotyping, but could not able to find any. Can anyone recommend papers related to this.

10 August 2023 4,838 1 View

How to distinguish good CAM assay eggs from those contamined? How to find out CAM assay eggs contaminated or not?

I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...

02 July 2023 9,319 1 View

How to distinguish good CAM assay eggs from those contaminated? How to find out CAM assay eggs contaminated or not?

I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...

02 July 2023 6,467 0 View

How to distinguish good CAM assay eggs from those contaminated? How to find out CAM assay eggs contaminated or not?

I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...

02 July 2023 4,050 0 View

L5178y TK+ cell line culturing?

Currently received this cell line L5178Y for some cell works. The prescribed media for the cell line was DMEM, but as it a suspension cell, the cells are maintained in RPMI media also. At the...

26 May 2023 2,216 0 View

Is 90% alcohol toxic to cancer cell lines?

I have to check the cytotoxicity of a drug using MTT assay. The drug is soluble in 90% alcohol, not in DMSO. Is 90% alcohol toxic to cells?

12 May 2023 7,759 3 View

DAPI staining without permeabilization is possible?

I have done DAPI staining in untreated and drug-treated EA carcinoma cells using 96-well plate. The procedure included only pbs washes, fixation with paraformaldehyde and incubation of each wells...

03 May 2023 9,064 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Does the gender of plants affect in the level of DPPH?

I have conducted research on the genus Salix, ad my results show that gender has a direct impact on the DPPH. Has anyone else obtained similar results or do you know of any articles that confirm this?

27 July 2024 5,144 1 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View