Cyclophosamide requires enzymatic activation by cytochrome P450 enzymes, before it displays its cytotoxic action via DNA alkylation and DNA cross-linking. Therefore, the value you obtain may not be relevant if you don't include an activating system.
@Philip G Penketh Thank you sir for your response. Do you have any reference paper supporting the statement?
S Shrivastav, K R Stone, D F Paulson, R A Bonar
Activation of cyclophosphamide for in vitro testing of cell sensitivity
Cancer Res. 1980 Dec;40(12):4443-5.
Philip G Penketh Hello sir! Hearty gratitude towards all of your responses. But, I couldn't an get open access to the paper you have mentioned. If you have and can share the pdf format of the paper, then it could be a great help.
I'm now retired and don't have access to most publications, however, if any of the authors are on ResearchGate, you could request it from them.
Good luck. Phil
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