How to analyze tube formation assay using Image J?

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Anurag Nishad

Hi Ameena Stellixir ,

1. Try to find show scale bar in your microscope software setting. You will get a scale bar in your captured images.

2. Draw a straight line with straight line tool on the scale bar (lengthwise) in the image and measure it (ctrl+M).

3. This will be the known distance value.

Nandaraj Taye

Hello, this tutorial video contains how to analyze tube formation using imagej

https://m.youtube.com/watch?v=sQLYoJxgUCk

This tutorial video contains how to set scale

https://m.youtube.com/watch?v=h6S_oXbt9RI