How to set scale bar in the image using image j? What to mention against "known distance" in the setting scale menu?
Hi Ameena Stellixir ,
1. Try to find show scale bar in your microscope software setting. You will get a scale bar in your captured images.
2. Draw a straight line with straight line tool on the scale bar (lengthwise) in the image and measure it (ctrl+M).
3. This will be the known distance value.
Hello, this tutorial video contains how to analyze tube formation using imagej
https://m.youtube.com/watch?v=sQLYoJxgUCk
This tutorial video contains how to set scale
https://m.youtube.com/watch?v=h6S_oXbt9RI
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