Please suggest some standard drug and its IC50 concentration which is applicable for CHO cell line.
As part of angiogenesis study, we did CAM assay with two replicates. The data we needed is of vascularization degree. But, the values in 0th and 24th hour first replicate is not similar to the...
30 October 2023 7,235 0 View
We are planning to do ELISA/ spectroscopy-based study. How to make it for time and dose-dependent assay, like OD values should be taken for every 20secs for 20 mins? Hence, how to calculate Vmax...
18 October 2023 9,335 0 View
I would like to read on papers related to CHO-K1 karyotyping, but could not able to find any. Can anyone recommend papers related to this.
10 August 2023 4,838 1 View
I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...
02 July 2023 9,318 1 View
02 July 2023 6,466 0 View
02 July 2023 4,049 0 View
Currently received this cell line L5178Y for some cell works. The prescribed media for the cell line was DMEM, but as it a suspension cell, the cells are maintained in RPMI media also. At the...
26 May 2023 2,216 0 View
I have to check the cytotoxicity of a drug using MTT assay. The drug is soluble in 90% alcohol, not in DMSO. Is 90% alcohol toxic to cells?
12 May 2023 7,758 3 View
I have done DAPI staining in untreated and drug-treated EA carcinoma cells using 96-well plate. The procedure included only pbs washes, fixation with paraformaldehyde and incubation of each wells...
03 May 2023 9,064 3 View
Planning to do DAPI staining using fluorescence microscopy. Thought of seeding the cells in 6-well plate. What all things should be considered while doing the assay? Does anyone have protocol for...
26 April 2023 3,868 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,105 4 View
How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.
06 August 2024 8,848 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 640 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View