For an in-vitro scratch assay to be conducted using a collagen sheet, what is the solubility of the collagen sheet and how can we prepare the working concentration of the sheet (i.e., 0.025%)?
Please suggest some standard drug and its IC50 concentration which is applicable for CHO cell line.
14 November 2023 7,586 0 View
As part of angiogenesis study, we did CAM assay with two replicates. The data we needed is of vascularization degree. But, the values in 0th and 24th hour first replicate is not similar to the...
30 October 2023 7,235 0 View
We are planning to do ELISA/ spectroscopy-based study. How to make it for time and dose-dependent assay, like OD values should be taken for every 20secs for 20 mins? Hence, how to calculate Vmax...
18 October 2023 9,335 0 View
I would like to read on papers related to CHO-K1 karyotyping, but could not able to find any. Can anyone recommend papers related to this.
10 August 2023 4,838 1 View
I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...
02 July 2023 9,319 1 View
02 July 2023 6,467 0 View
02 July 2023 4,050 0 View
Currently received this cell line L5178Y for some cell works. The prescribed media for the cell line was DMEM, but as it a suspension cell, the cells are maintained in RPMI media also. At the...
26 May 2023 2,216 0 View
I have to check the cytotoxicity of a drug using MTT assay. The drug is soluble in 90% alcohol, not in DMSO. Is 90% alcohol toxic to cells?
12 May 2023 7,759 3 View
I have done DAPI staining in untreated and drug-treated EA carcinoma cells using 96-well plate. The procedure included only pbs washes, fixation with paraformaldehyde and incubation of each wells...
03 May 2023 9,064 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View