Competent yeast cell preparation makes use of PEG which has been implicated in bringing the cells and the plasmid in close proximity for effective transformation. Is there any proper evidence for this? What are the alernatives for PEG?
It is said to be "molecular crowding" effect.
Presence of high concentration of macromolecules (e.g., PEG) can constrain the "movable space" of other macromolecule (e.g., DNA), which can give a same effect as increased concentration of the second macromolecule (DNA).
This is the theory, but I am not sure if the mechanism is experimentally proven for yeast transformation.
Dear Gayatri Mohanan
Pls. find the attached files and the references, good luck
Hoping this will be helpful to your project
Regards
Prof. Houda Kawas
http://pubs.acs.org/doi/abs/10.1021/bi00345a005
http://jes.ecsdl.org/content/145/10/3472.short
http://www.tandfonline.com/doi/abs/10.3109/08982109409037068
http://www.sciencedirect.com/science/article/pii/0022072892804209
https://www.drugbank.ca/drugs/DB09287
https://www.ncbi.nlm.nih.gov/pubmed/6805940
https://en.wikipedia.org/wiki/Polyethylene_glycol
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3056089/
Also
http://www.biotechniques.com/BiotechniquesJournal/2013/January/A-novel-method-for-genetic-transformation-of-yeast-cells-using----oligoelectrolyte-polymeric-nanoscale-carriers/biotechniques-339017.html?pageNum=3
https://link.springer.com/article/10.1007%2Fs00294-008-0204-z
https://biology.stackexchange.com/questions/1995/why-is-peg-important-for-efficient-yeast-transformation
https://books.google.com/books?id=TxCQlmasQh8C&pg=PA856&lpg=PA856&dq=polyethylene+glycol+mode+of+action+with+yeast+cells&source=bl&ots=BB3aWE8h2P&sig=FQ_FNp6RmWabr1CZyoYKDoFxWVY&hl=ar&sa=X&ved=0ahUKEwjT55zAtNnUAhXqC5oKHd18DtwQ6AEISzAF#v=onepage&q=polyethylene%20glycol%20mode%20of%20action%20with%20yeast%20cells&f=false
http://jcb.rupress.org/content/jcb/96/1/151.full.pdf
http://pubs.acs.org/doi/abs/10.1021/jf3010405?journalCode=jafcau
http://onlinelibrary.wiley.com/doi/10.1002/yea.2951/pdf
https://books.google.com/books?id=ZJ4H6wbVfWMC&pg=PA22&lpg=PA22&dq=polyethylene+glycol+mode+of+action+with+yeast+cells&source=bl&ots=HINkfiQuEH&sig=VmE3k31qhoIaxrgAo9epqblW5oI&hl=ar&sa=X&ved=0ahUKEwjT55zAtNnUAhXqC5oKHd18DtwQ6AEIaTAJ#v=onepage&q=polyethylene%20glycol%20mode%20of%20action%20with%20yeast%20cells&f=false
Article Studies on the transformation of intact yeast-cells by the L...
Hello Gayatri
Some helpful references on attachment 1
https://pubmedcentralcanada.ca/pmcc/articles/PMC214267/pdf/jbacter00215-0476.pdf
2
http://capricorn.bc.edu/bi204/wp-content/uploads/2013/08/12-yeast-transformation-2013.pdf
6
http://www.sigmaaldrich.com/technical-documents/protocols/biology/yeast-transformation-protocols.html
9
http://www.biolchem.ucla.edu/labs/grunstein/LiAc-Mediated%20Yeast%20Transformation.html
11
http://www.biotechniques.com/multimedia/archive/00003/BTN_A_05381BM04_O_3873a.pdf
12
https://www.biotechniques.com/multimedia/archive/00010/01304rv02_10885a.pdf
14
https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n1-8/442
15
https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014
17
https://www.ozyme.fr/gammes/tak/pdf/clo_easy_yeast_plasmid_isolation.pdf
20
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=23&cad=rja&uact=8&ved=0ahUKEwjh3NXi6rHVAhXDDcAKHSgcAqc4FBAWCCswAg&url=https%3A%2F%2Fwww.degruyter.com%2Fdownloadpdf%2Fj%2Fbiol.2008.3.issue-4%2Fs11535-008-0040-7%2Fs11535-008-0040-7.xml&usg=AFQjCNGG_p7Dft-QCz40HJCsZ_TgN6DgQw
Article Transformation of yeast by the LiAc/ss carrier DNA/PEG method
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