I would like to do TA muscle sectioning using vibratome. I fixed the muscles for 2 h in 4% PFA. After this step, I placed in PBS for weeks. How long can these tissues last, any suggestions?
we use lung, mouse and human, which we store after fixation in pbs with a some PS in the fridge. Stainings turned out fine even after about 6 weeks of storage for tissue pieces and 8 weeks for already cut vibratome slices.
I never tried to store them longer though so I can't tell you what is the maximum.