Muhammad Sufyan Tahir

7 Questions 11 Answers 0 Followers

Questions related from Muhammad Sufyan Tahir

Any tool to scan the promoter sequences of genes to find already known stress related transcription transcription factors elements??

I need to analyze genes and their response towards ABA and other a biotic stresses. I wanna check if there are any ABA related or other Transcription factors elements in the promoter sequence of...

13 November 2019 1,618 3 View

Why am I getting false positive colonies after electroporation of agrobacterium GV3101 with my constructs?

I have done many experiments and always i get colonies on LB-agar plates (Selection is Kana 50, Genta 25), But colony PCR always appears negataive with very good bands in positive control.

21 October 2019 7,141 7 View

Why my transformed GV3101 agrobacterium glycerol stock is showing negative PCR results?

I did gateway cloning and transferred my constructs into GV3101. I did several PCR confirmations of GV3101 and made glycerol stocks and stored at -80. After about 3-4 months. I am unable to...

05 August 2019 6,199 3 View

Can we perform perform Co immunoprecipitation or other protein-protein interaction relevant assays in the same plant in which we are doing studies?

I am working with 4 histone deacetylase genes (ABCD) to study their interactions with each other against stress environment. I have read through different assays (like Y2H, BiFC, Co...

02 August 2018 2,213 3 View

How we can use T-DNA insertion mutant plants of Arabidopsis to study protein protein interaction and function?

I am currently working on Histone deacetylases (HDACs) in Arabidopsis under certain stress conditions. My objective is to study the interaction of certain HDACs among each other in regulating the...

11 February 2018 8,222 0 View

How we can compare two full length genome or even DNA sequences of one complete chromosome from two species using online or offline tools?

I use different online tools like LAST, BLAST, VISTA etc, and want results in figures but all these tools give error regarding the size of data. like size of data is greater or somewhat else....

05 February 2016 492 6 View

Will you please provide me some publications regarding the arboreum cotton, its acclimatization to soil from MS media?

as i am working on arboreum cottom transformation, nut i am feeling some disturbances in plants acclimatization into soil from MS media. also there is white light set in the culture room. you...

01 January 2016 9,632 3 View