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Questions related from Muhammad Sufyan Tahir
I need to analyze genes and their response towards ABA and other a biotic stresses. I wanna check if there are any ABA related or other Transcription factors elements in the promoter sequence of...
13 November 2019 1,471 3 View
I have done many experiments and always i get colonies on LB-agar plates (Selection is Kana 50, Genta 25), But colony PCR always appears negataive with very good bands in positive control.
21 October 2019 7,083 7 View
I did gateway cloning and transferred my constructs into GV3101. I did several PCR confirmations of GV3101 and made glycerol stocks and stored at -80. After about 3-4 months. I am unable to...
05 August 2019 6,135 3 View
I am currently working on Histone deacetylases (HDACs) in Arabidopsis under certain stress conditions. My objective is to study the interaction of certain HDACs among each other in regulating the...
11 February 2018 8,168 0 View
I use different online tools like LAST, BLAST, VISTA etc, and want results in figures but all these tools give error regarding the size of data. like size of data is greater or somewhat else....
05 February 2016 446 6 View
as i am working on arboreum cottom transformation, nut i am feeling some disturbances in plants acclimatization into soil from MS media. also there is white light set in the culture room. you...
01 January 2016 9,587 3 View