Gayatri Mohanan

3 Questions 1 Answers 0 Followers

Questions related from Gayatri Mohanan

When do we see very thick 5s rRNA band and faint 28s and 18s rRNA for yeast total RNA isolation?

I am trying to isolate total RNA from yeast lysate. The lysis is done using glass-bead beating. The lysate is collected and precipitated overnight with sodium acetate and ethanol. The precipitant...

31 July 2021 3,463 2 View

I need to isolate nucleus from budding yeast with high purity. I need intact nucleus. Can someone suggest any protocol that I can use?

I need high purity nuclear fractions from budding yeast cultures for biochemical assays. I am able to get spheroplasts but getting the nucleus out without damaging the nucleus and without...

30 December 2017 3,866 0 View

Why is polyethylene glycol (PEG) used for the preparation of competent yeast cells? Does it affect the cellular osmolarity of yeasts?

Competent yeast cell preparation makes use of PEG which has been implicated in bringing the cells and the plasmid in close proximity for effective transformation. Is there any proper evidence for...

21 June 2017 9,420 24 View