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I am trying to isolate total RNA from yeast lysate. The lysis is done using glass-bead beating. The lysate is collected and precipitated overnight with sodium acetate and ethanol. The precipitant...
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I need high purity nuclear fractions from budding yeast cultures for biochemical assays. I am able to get spheroplasts but getting the nucleus out without damaging the nucleus and without...
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Competent yeast cell preparation makes use of PEG which has been implicated in bringing the cells and the plasmid in close proximity for effective transformation. Is there any proper evidence for...
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