Hi,
Is there any software available to delete certain regions of the stem loop structure? Please advise
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Would like to have suggestions on books I could read having recent developments in Green Buildings.
28 September 2020 2,293 7 View
I have developed a 3 bay 5 story 2D frame in Opensees but I have problems with element load and application of seismic load in Opensees using an acceleration file. I always got an error namely...
27 September 2020 5,885 4 View
Dear All, I kindly ask about useful references and papers related to build the 3D-geometry for a nanoparticle embedded refractive index based planar waveguides. All the relevant literature do not...
25 September 2020 9,136 2 View
I have been using NetSim simulator and now I want to try to simulate a DOS attack in 5G. Can someone guide me on the best way to implement this in NetSim?
27 May 2020 9,187 6 View
1. They claim themseleves as web of science and it is delisted from scopus sources (its been 3 years they are delisted) 2. web of science they claim annual publication (in reality they are...
27 January 2020 6,544 5 View
I am trying to generate the be.dat file using the gen_be facility of WRF. I have 12 hour and 24 hour outputs from WRF at 00 UTC and 12 UTC for a month. When I try to run gen_be_wrapper.ksh on my...
15 January 2020 244 2 View
In compressive sensing of images Digital MicroMirror Device (DMD) is used to implement the sensing matrix. DMD works in two states, on and off. This can implement a binary sensing matrix. How is a...
05 November 2019 2,096 3 View
Do anyone know is it necessary to use low attachement plate instead can I use bacterological petri dish ?
10 October 2019 5,587 2 View
In my paper, I am only testing the effects of affective commitment and normative commitment but not continuous commitment. I keep having a problem in my paper when I am creating a hypothesis. Can...
18 September 2019 3,942 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...
02 March 2021 728 2 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View