Hi,
Is there any software available to delete certain regions of the stem loop structure? Please advise
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,781 2 View
I am working on cold spray technology and currently doing validation for discrete phase modelling. The total mass flow rate and initial velocity of the particles is not mentioned in the paper...
08 April 2024 9,612 4 View
I am currently validating a paper on Cold Spray technology and simulating the particle flow using 'Discrete Particle Modelling'. I got the particle tracks and the particle history data, but...
03 April 2024 9,465 2 View
I will be using the Himedia PCR cleanup protocol and kit, so if anyone has any hacks to reduce the smear, do let me know. I will be sending samples urgently for sequencing. The sequencing is...
03 January 2024 6,634 3 View
I would want to know more about the area. Any leads would be helpful. Thank you.
21 December 2023 3,500 4 View
Although there are several papers regarding EPS measurement in sludge, it is quite confusing. Like exactly what need to be measured , and how is that calculated. I would appreciate if someone...
24 November 2023 7,766 4 View
We grow the microbe in complex sugar media, spin it, take the supernatant, and add buffer and water. In the case of a test, we add the substrate and incubate it before adding DNSA, bringing it to...
05 October 2023 6,302 0 View
in terms of abroad and in India
20 August 2023 3,885 10 View
I have a hydrogel that visually looks like a gel (it is viscoelastic similar to mucus). When I do rheology on it, however it tends to show liquid-like behaviour (G''>G'), at higher angular...
17 July 2023 4,455 5 View
I need to calculate oxygen exchange current density using polarization resistance.
07 June 2023 9,235 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,619 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,365 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,724 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View