Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

In the paper mentioned can you help me to find the finite difference of equation no 19.?

In the paper "Magnetometer-Only Attitude Determination Using Novel Two-Step Kalman Filter Approach" in equation 19 do I need to complete quaternion multiplication first and then do simple...

15 June 2020 7,522 4 View

How to align reverse and forward DNA sequences in chromus software ??

Need detailed procedure to align DNA seq. before performing BLAST.

09 January 2019 7,584 3 View

For detecting single nucleotide mutation, do we use traditional PCR or qPCR?

I have got a deletion of single base pair thymine at 3744 position in BRCA1 gene, which PCR technique should I use to detect our gene mutation ?

05 March 2018 3,492 3 View

How to calculate grafting % for two polymers?

Hello, I am performing grafting of two polymers using microwave assisted method. I want to know that what is the correct method to assess and calculate grafting % among the two polymers. Is...

04 February 2018 6,874 3 View

How can you ensure that the drug is completely separated from liposomes?

After doing either sephadex column or ultrafiltration. How do you ensure that entire unentrapped drug is removed from liposomes? Will there be any extra peak in DLS?

10 January 2018 1,293 6 View

Can you suggest best cost effective CO2 incubator selling company for animal cell culture?

Hi I am looking for best cost effective CO2 incubator for animal cell culture facility. There are lot of models by various companies with different features. As such, so many options and hence, a...

20 August 2017 1,957 7 View

I want to study rearrangement of ROS 1 and RET genes in FFPE sample?

24 December 2016 4,778 1 View

What is the actual molecular weight of CD63 for exosome identification?

Hii, I always get variable result for CD63 immunoblot from exosomal protein as well as cellular protein of RAW cell line macrophages.I found some paper where it mentioned that CD63 has alternative...

29 November 2016 4,536 0 View

Is there any labs in India working with Brachypodium distachyon? Do anyone have its seeds ?

29 January 2016 469 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View