I tried to immobilize CTAC capped gold nanotriangle which is synthesized by seed mediated growth method by various method. (1) Glass coverslip was cleaned by: 1N NaOH, acetone, methanol, milli-q water sonicated 10 min in each, rinsed copiously by DI water and dried using Ar flow. This glass coverslip was functionalized by applying APTMS vapor. 400 microliter AuNTs were immobilized. (2) Precleaned glass coverslip was immersed in 10% V/V APTMS solution in methanol. But i could not achieve result. Please suggest me how can i immobilize AuNTs glass coverslip.